Immunology

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    Protein- and Sequencing-based Massively Parallel Single-cell Approaches to Gene Expression Profiling
    Authors:  Xuhuai Ji, Igor Goncharov, Miao Zhao, Molly Miranda and Holden T. Maecker, date: 02/05/2019, view: 4917, Q&A: 0
    Understanding cellular diversity and dynamics in immune cells are critical for elucidating mechanisms of diagnosis, response to therapeutics, and patient prognosis. Our goal is to apply a technically simple approach for gene expression cytometry ...
    In vivo BrdU Incorporation and Detection in Mouse
    Author:  Zheng Liu, date: 06/05/2011, view: 34704, Q&A: 0
    BrdU (Bromodeoxyuridine or 5-bromo-2’-deoxyuridine) is a synthetic nucleoside that is incorporated into DNA by proliferating cells. This protocol is to be used to incorporate and detect BrdU in murine plasma cells. The plasma cells described in this ...
    Immunofluorescent Staining of Murine Tissue
    Author:  Zheng Liu, date: 05/05/2011, view: 15790, Q&A: 0
    Renal immune complex deposition and leukocyte infiltration are characteristic of lupus nephritis in human patients and lupus-prone mice. This protocol describes how to stain frozen sections of murine kidney to study these features using fluorescent ...



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