Biochemistry

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Western Blot in Maize

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    Crosslinking and Immunoprecipitation in Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 11908, Q&A: 0
    Immunoprecipitation (IP) is a routine method to detect protein binding and interactions. But the weak binding between two proteins is often hard to detect during regular IP procedure. This protocol offers a crossliking and IP combination method to ...
    Protein-ligand Binding Assay by Liquid Chromatography-Mass Spectrometry
    Author:  Xiyan Li, date: 08/20/2011, view: 14381, Q&A: 0
    Protein-small molecule binding coefficients are determined by quantitative LC-MS in this method. Traditional biochemical plot is consequently used to set up the binding curve between a known protein and a small molecule (<1000 da) to determine the affinity constant (kd) and the stoichiometry (bmax). da)="" to="" determine="" the="" affinity="" constant="" (kd)="" and="" the="" stoichiometry=""> ...
    Rabbit IgG Conjugation to Dynabeads
    Author:  Xiyan Li, date: 05/05/2011, view: 13910, Q&A: 1
    This method couples rabbit IgG (or any other proteins serve as affinity reagent) to the surface of magnetic beads (Brand name: Dynabeads). The amine and thiol groups of amino acid residues on the protein are covalently linked with the epoxy group on ...
    Affinity Purification of Yeast Protein-interacting Metabolites for ESI-MS Analysis
    Author:  Xiyan Li, date: 04/20/2011, view: 13016, Q&A: 5
    The method described here can be used to discover in vivo protein-metabolite interactions. Metabolite-protein complexes are purified from yeast cell lysates by an affinity tag that recognizes the protein of interest. The protein-bound metabolites ...
    A General EMSA (Gel-shift) Protocol
    Author:  Ran Chen, date: 02/05/2011, view: 51439, Q&A: 3
    An electrophoretic mobility shift assay (EMSA), also referred to as mobility shift electrophoresis, a gel shift assay, gel mobility shift assay, band shift assay, or gel retardation assay, is a common technique used to study protein-DNA or ...