Plant Science

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    Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue
    Authors:  Josh Strable, Jeffrey R. Yen, Michael J. Scanlon and Anne W. Sylvester, date: 05/05/2020, view: 354, Q&A: 0
    Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...
    Staining Maize Epidermal Leaf Peels with Toluidine Blue O
    Authors:  Marschal A. Bellinger, Sukhmani K. Sidhu and Carolyn G. Rasmussen, date: 04/20/2019, view: 3471, Q&A: 0
    Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
    Microscopic Observation of Subcellular GFP-tagged Protein Localization in Rice Anthers
    Authors:  Seijiro Ono and Ken-Ichi Nonomura, date: 08/05/2018, view: 2654, Q&A: 0
    This protocol demonstrates a simple method to determine the subcellular localization of fluorescence-tagged proteins on the vibratome sections of rice developing anthers. If a cell type-specific promoter is used to drive the tagged protein-encoding ...
    MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
    Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 6001, Q&A: 0
    Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...
    Histostaining for Tissue Expression Pattern of Promoter-driven GUS Activity in Arabidopsis
    Author:  Xiyan Li, date: 07/05/2011, view: 26477, Q&A: 3
    Promoter-driven GUS (beta-glucuronidase) activity is the most commonly used technique for tissue-specific expression patterns in Arabidopsis. In this procedure, GUS enzyme converts 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) to a blue product. ...
    Arabidopsis Pollen Tube Aniline Blue Staining
    Author:  Yongxian Lu, date: 06/20/2011, view: 23423, Q&A: 1
    The aim of this experiment is to track pollen tube growth in vivo in the female tissues after pollination. This can be used to phenotype pollen germination, tube growth and guidance, and reception.



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