Developmental Biology

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    Isolation of Murine Brain and Lung Microvascular Endothelial Cells
    Authors:  Ailing Zhang, Shogo Sawaguchi, Entzu Wan, Mitsutaka Ogawa and Tetsuya Okajima, date: 08/20/2018, view: 2910, Q&A: 0
    This protocol describes how to isolate murine endothelial cells from newborn mice brain and 3-month-old mice lung by modifying the original protocols (Sobczak et al., 2010; Ruck et al., 2014). We have used the protocol to analyze ...
    Cell Culture Transfection for Production and Purification of Wnt Ligands
    Author:  Lili Jing, date: 01/20/2012, view: 18216, Q&A: 0
    Wnt ligand proteins are extremely difficult to purify and enrich in vitro. This protocol uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect it in vitro for further ...



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