Molecular Biology

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    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 98, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
    Agrobacterium-mediated Transformation of Nicotiana tabacum cv. Xanthi Leaf Explants
    Authors:  Ana Lilia Rosales-Campos and Abel Gutiérrez-Ortega, date: 01/20/2019, view: 984, Q&A: 0
    Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is Nicotiana tabacum ...
    Nuclear Transformation of Chlamydomonas reinhardtii by Electroporation
    Author:  Tyler M Wittkopp, date: 05/05/2018, view: 2970, Q&A: 0
    The unicellular green alga Chlamydomonas reinhardtii is an important model organism for studying photosynthesis, acclimation to abiotic stress, cilia biology, and many other biological processes. Many molecular biology tools exist for ...
    Transfection of S2 Cell with DNA Using CellFectin Reagent
    Author:  Darran G. Cronshaw, date: 03/05/2012, view: 16455, Q&A: 0
    This method provides a step-by-step guide to transfecting Drosophila S2 cells with the pRmHA-3 (or similar) vector with insert of choice (in this case SDF-1β-FLAG) and generating a stable cell line. This cell line is then capable of producing the ...
    Simplified Agrobacterium-mediated Transformation of Arabidopsis thaliana
    Author:  Li-Qing Chen, date: 03/05/2011, view: 16193, Q&A: 0
    Acquiring transgenic lines is fundamental strategy for plant gene/protein function study. How to successfully obtain the transgenic lines is a big matter with transformation methods. Now Arabidopsis floral dip transformation method is more ...
    Injecting Zebrafish Embryos
    Author:  Lili Jing, date: 01/20/2011, view: 14901, Q&A: 0
    Zebrafish is convenient for transient genetic manipulations such as morpholino-mediated gene knockdown, DNA/mRNA-based genetic overexpression. These genetic approaches do not involve the challenge of making transgenic animals, and their effect can ...