Molecular Biology


    Using Seed Chipping to Genotype Maize Kernels
    Authors:  Alison M. Mills, Lindy A. Allsman, Sareen Leon and Carolyn G. Rasmussen, date: 03/20/2020, view: 1841, Q&A: 0
    Zea mays (maize) is an important model organism for studying monocot growth and development. Genotyping maize in the greenhouse or field can be time consuming and costly. Here, we describe a method to remove or chip a small amount of the ...
    Growing and Pollinating Maize
    Authors:  Sarah Hake and China Lunde, date: 05/05/2018, view: 3740, Q&A: 0
    [Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
    Genotyping for Single Zebrafish (Fin Clip) or Zebrafish Embryo
    Author:  Lili Jing, date: 02/05/2012, view: 26148, Q&A: 0
    Zebrafish is increasingly used a genetic model organism in biomedical studies. This protocol provides a detailed procedure about the identification of the genotype of an adult zebrafish or a zebrafish embryo.
    Genotyping Transgenic Zebrafish Using Genomic DNA Extracted from Clutch of Embryos
    Author:  Lili Jing, date: 02/05/2012, view: 13425, Q&A: 0
    Transgenic zebrafish are very useful genetic tools to study various biological processes. Identification the right transgene founder and the subsequent transgenic animals are always tedious and time consuming. This protocol provides a relatively ...
    Site-directed Mutagenesis Using Dpn1
    Author:  Lili Jing, date: 02/05/2011, view: 21171, Q&A: 1
    Site-directed mutagenesis is an important and widely used tool in molecular biology to generate specific changes in the DNA sequence of a given gene/genome. The protocol described here is for making nucleotide changes at specific loci in a large ...

    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.