Microbiology

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    Phenol-based Extraction of RNA from Chlamydomonas reinhardtii
    Authors:  Emanuel Sanz-Luque and Amaury de Montaigu, date: 07/05/2018, view: 3253, Q&A: 0
    RNA extraction is a basic procedure in molecular biology and a wide variety of commercial kits are available. Some of these kits have been successfully used in Chlamydomonas. However, in some cases RNA quality and quantity may be ...
    qPCR of Yeast ChIP DNA
    Author:  Wei Zheng, date: 09/20/2011, view: 15088, Q&A: 1
    This protocol is adapted from Chris Yellman's personal notes for qPCR of yeast ChIP DNA to verify binding sites already identified by microarray/sequencing analysis. In addition, qPCR is a quick way to assay the quality of a ChIP sample ...
    Yeast in situ Hybridization
    Author:  Wei Zheng, date: 09/20/2011, view: 10389, Q&A: 0
    This protocol describes the procedure to label a certain mRNA species in yeast cells with fluorescence probes and prepare slides for visualization under microscope. Probe design/synthesis and microscopy operation are not covered.
    RNA Preparation for Microarray Experiments
    Author:  Bio-protocol Editor, date: 01/20/2011, view: 13900, Q&A: 0
    This protocol describes a simple and relatively general method to extract total RNA from a yeast culture using the Qiagen RNeasy kit. In it, total RNA is treated with DNase I and cleaned up to be suitable for microarray experiments. Therefore, this ...



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