Immunology

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    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-histone Antibodies
    Author:  Zheng Liu, date: 06/20/2012, view: 9775, Q&A: 0
    Autoimmune disease is characterized with the break of tolerance against self antigens. Anti-histone antibodies can be found in the majority of patients with system lupus erythematosus (SLE) and a number of lupus-prone mouse strains. This protocol ...
    ELISA Measurement of Mouse IL-2
    Author:  Guo N. Huang, date: 01/05/2012, view: 9503, Q&A: 0
    Interleukin-2 (IL-2) is a cytokine secreted by T cells that is essential for immune system activation. This protocol is routinely used for quantification of IL-2 concentration in the supernant of cultured lymphocytes under various stimulations and ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Murine Anti-dsDNA Antibodies
    Author:  Zheng Liu, date: 02/20/2011, view: 13185, Q&A: 0
    ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine anti-dsDNA antibodies. Circulating anti-dsDNA antibody is a hallmark of SLE both in human patients and in ...
    Quantitative Enzyme-linked Immunosorbent Assay (ELISA) to Measure Serum Levels of Total Murine Immunoglobulin
    Author:  Zheng Liu, date: 02/05/2011, view: 13060, Q&A: 0
    ELISA is an easy and relatively sensitive way to measure protein concentration. This protocol describes how to measure serum levels of murine total immunoglobulin of various isotypes. This protocol was developed or modified in Dr. Anne Davidson’s ...



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