Molecular Biology


    VSVG Psudotyped Retrovirus Production
    Author:  Jia Li, date: 12/05/2011, view: 16487, Q&A: 0
    Retrovirus vector pseudotyped with vesicular stomatitis virus G (VSV-G) protein has been proven to exhibit high efficiency to deliver genes in a variety of cells. Efficiency is affected by relative cell growth rate and phosphatidylserine ...
    RNA Interference (RNAi) by Bacterial Feeding
    Author:  Fanglian He, date: 04/20/2011, view: 25758, Q&A: 0
    There are 3 ways to perform RNAi in worms: microinjection, soaking and feeding. In the feeding protocol, RNAi is induced by cultivating worms on bacteria expressing gene-specific dsRNA. dsRNA is expressed in E. coli and ingested by worms. ...
    Lentivirus Production
    Author:  Nabila Aboulaich, date: 03/05/2011, view: 48038, Q&A: 6
    Lentivirus is a common tool used to introduce a gene into mammalian or other animal cells.This protocol is to produce lentivirus stocks from hairpin-pLKO.1 plasmid.
    siRNA Transfection of Mouse Bone Marrow-derived Macrophages
    Author:  Ran Chen, date: 02/05/2011, view: 21973, Q&A: 2
    Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in ...

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