Cell Biology

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    Protocol to Determine Mitotic Index by FACS
    Author:  Hui Zhu, date: 03/20/2012, view: 16208, Q&A: 0
    Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
    pH3 Antibody Staining Protocol for Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 23049, Q&A: 0
    Phosphorylation of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Staining for phosphor histone H3 is a good indicator for the proliferation of the interested cells. This protocol provides a method to ...
    Western Blot for Detecting Phosphorylated STAT3
    Author:  Huagang Zhang, date: 08/20/2011, view: 21665, Q&A: 0
    The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. STAT3 is ...



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