Cell Biology

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    Transient Transformation of Maize BMS Suspension Cells via Particle Bombardment
    Authors:  Liang-Zi Zhou and Thomas Dresselhaus, date: 12/05/2019, view: 1352, Q&A: 0
    Maize is one of the most important crop species and serves also as a model plant for grass research. A major bottleneck in maize research is stable transformation, which is both time and cost consuming, but also a technical challenge for most labs ...
    Isolation and Efficient Maize Protoplast Transformation
    Authors:  Lina Gomez-Cano, Fan Yang and Erich Grotewold, date: 08/20/2019, view: 2588, Q&A: 0
    Establishing plant gene function and the study of gene expression regulation often require that genes (or gene variants) are introduced into plants and their activity somehow assayed. Some plants (e.g., Arabidopsis) are easily ...
    A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
    Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 14800, Q&A: 1
    This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
    Cell Culture Transfection for Production and Purification of Wnt Ligands
    Author:  Lili Jing, date: 01/20/2012, view: 18545, Q&A: 0
    Wnt ligand proteins are extremely difficult to purify and enrich in vitro. This protocol uses Wnt11r protein as an example to illustrate how to use 293T cells to produce secreted Wnt11r and collect it in vitro for further ...
    Making Yeast Competent Cells and Yeast Cell Transformation
    Author:  Yongxian Lu, date: 07/20/2011, view: 38587, Q&A: 3
    This is a quite simple but reliable protocol to make very high transformation efficiency yeast competent cells. By express your gene of interest, protein function can be studied in yeast cells.



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