Biochemistry

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    Labeling Protein with Thiol-reactive Probes
    Author:  Qingrong Yan, date: 06/05/2011, view: 10738, Q&A: 1
    This protocol is used to label protein or peptide with a maleinmide or iodoacetamide conjugated fluorescent probe through the free cysteine.
    Metabolic Labeling of Yeast Proteins
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 11653, Q&A: 0
    Proteins of Saccharomyces cerevisiae can be metabolically labeled with (35) methionine. After labeling, a protocol is described for the mechanical disruption of yeast cells or conversion to spheroplasts, with subsequent lysis before ...



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