Biochemistry

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    Very Low Molecular Weight Proteins Electrophoresis Protocol
    Authors:  César E. Rivera, José D. Rosales, Juan C. Freites-Perez and Eliaira Rodriguez, date: 11/20/2018, view: 5330, Q&A: 1
    The electrophoresis is the most used technique to separate proteins and usually the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) proposed by Laemmli is the prefered since it can diferentiate large size proteins, but for very ...
    SDS-PAGE for Silk Fibroin Protein
    Author:  Yu-Qing Zhang, date: 10/20/2018, view: 4561, Q&A: 0
    The method and detailed procedure of SDS-PAGE for silk proteins are exactly the same as for other proteins, but the electrophoresis profile of silk protein is often unsatisfactory. The main reason is that their molecular masses are too large, and ...
    Rapid Coomassie Protein SDS-gel Staining
    Author:  Qingrong Yan, date: 06/20/2011, view: 15785, Q&A: 0
    This is a fast gel-staining protocol (within 10 min) compared to the conventional coomassie staining with the same detecting sensitivity.
    Laemmli-SDS-PAGE
    Author:  Fanglian He, date: 06/05/2011, view: 136232, Q&A: 12
    Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is used to separate proteins with relative molecular mass no smaller than 10 KD. Very small proteins (<10 KD) are difficult to resolve due to low ability of binding to SDS, ...
    Coomassie Blue Staining
    Author:  Fanglian He, date: 06/05/2011, view: 72477, Q&A: 0
    Coomassie staining is able to detect protein bands containing about 0.2 μg or more protein. For low abundant protein, silver staining (www/silver staining) is a better choice since it is about 100-fold more sensitive than Coomassie staining.
    Silver Staining
    Author:  Nabila Aboulaich, date: 02/05/2011, view: 29719, Q&A: 1
    Protocol for Whole Cell Lysis of Yeast
    Author:  Bio-protocol Editor, date: 01/05/2011, view: 36207, Q&A: 5
    This protocol describes how to perform lysis on whole yeast cell samples using NaOH. The lysed cells can then be used for downstream applications such as the extraction of total proteins.



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