Plant Science

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    Stable Transformation of Arabidopsis thaliana Cell Suspension Cultures: A Case Study for the Overexpression of The COI1 Receptor
    Cell suspension cultures have been studied for decades to produce natural molecules. However, the difficulty in generating stably transformed cell lines has limited their use to produce high value chemicals reproducibly and in elevated quantities. ...
    Optimize Highly Efficient Genetic Transformation Method of Spring Wheat (Triticum aestivum L.) Cultivars Using Mature Embryo
    Authors:  Dongjin Kim and Megan Hager, date: 11/20/2020, view: 981, Q&A: 0
    Genetic engineering is a powerful tool to develop desired traits in crop plants to make the crops suitable for future demand and changing environments. For the genetic engineering of wheat cultivars, the development of efficient transformation and ...
    Agrobacterium-mediated Transformation of Sweet Basil (Ocimum basilicum)
    Authors:  Natasha Navet and Miaoying Tian, date: 11/20/2020, view: 2098, Q&A: 1

    Sweet basil (Ocimum basilicum) is a popular herb with high economic value and is currently threatened by a severe oomycete disease. An efficient transformation method is a prerequisite for gene functional analysis to accelerate molecular breeding

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    Efficient Agrobacterium-mediated Transformation of the Elite–Indica Rice Variety Komboka
    Genetic transformation is crucial for both investigating gene functions and for engineering of crops to introduce new traits. Rice (Oryza sativa L.) is an important model in plant research, since it is the staple food for more than half of ...
    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 4077, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
    Agrobacterium-mediated Transformation of Nicotiana tabacum cv. Xanthi Leaf Explants
    Authors:  Ana Lilia Rosales-Campos and Abel Gutiérrez-Ortega, date: 01/20/2019, view: 7953, Q&A: 0
    Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is Nicotiana tabacum ...
    Visualization and Quantification of Cell-to-cell Movement of Proteins in Nicotiana benthamiana
    Authors:  Mila C Blekemolen, Mariliis Tark-Dame and Frank L.W Takken, date: 12/20/2018, view: 4448, Q&A: 0
    Cell-to-cell movement of proteins through plasmodesmata is a widely-established mechanism for intercellular signaling in plants. Current techniques to study intercellular protein translocation rely on single-cell transformation using particle ...
    Generation and Selection of Transgenic Olive Plants
    Authors:  Elena Palomo-Ríos, Sergio Cerezo, Jose Ángel Mercado and Fernando Pliego-Alfaro, date: 11/20/2017, view: 6507, Q&A: 0
    Olive (Olea europaea L.) is one of the most important oil crops in the Mediterranean basin. Biotechnological improvement of this species is hampered by the recalcitrant nature of olive tissue to regenerate in vitro. In previous ...
    Use of Geminivirus for Delivery of CRISPR/Cas9 Components to Tobacco by Agro-infiltration
    Authors:  Kangquan Yin, Ting Han and Yule Liu, date: 04/05/2017, view: 11118, Q&A: 0
    CRISPR/Cas9 system is a recently developed genome editing tool, and its power has been demonstrated in many organisms, including some plant species (Wang et al., 2016). In eukaryotes, the Cas9/gRNA complexes target genome sites specifically ...
    Multiplexed GuideRNA-expression to Efficiently Mutagenize Multiple Loci in Arabidopsis by CRISPR-Cas9
    Authors:  Julia Schumacher, Kerstin Kaufmann and Wenhao Yan, date: 03/05/2017, view: 9309, Q&A: 0
    Since the discovery of the CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein (Cas) as an efficient tool for genome editing in plants (Li et al., 2013; Shan et al., 2013; Nekrasov et al., ...



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