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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Plant Science
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Arabidopsis
Stomatal Opening and Closing Experiment
Author:
Zhixin Zhao
,
date:
05/20/2011,
view:
16567,
Q&A:
1
Stomata pores are mainly localized in the lower epidermis of plant leaves and transpirational water loss occurs through these pores. ABA inhibits light-promoted stomatal opening and promotes stomatal closure. This protocol describes how to measure ...
Arabidopsis
Pollen Tube Germination
Author:
Xiyan Li
,
date:
05/20/2011,
view:
17757,
Q&A:
0
This method uses a PEG-supplemented liquid solution to germinate separated
Aradidopsis
pollen. It thus eliminated the need for humidity control.
Pollen Fertility/viability Assay Using FDA Staining
Author:
Xiyan Li
,
date:
05/20/2011,
view:
27383,
Q&A:
3
Pollen grains can be fertile or sterile by nature. This method stains pollen grains for an enzyme as the vital indicator of membrane integrity. Only fertile grains fluoresce under microscopic examination.
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