Plant Science

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    Barley Transformation by Particle Bombardment Using Callus
    Author:  Sung-Yong Kim, date: 06/05/2020, view: 1648, Q&A: 0
    The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...
    Transient Transformation of Maize BMS Suspension Cells via Particle Bombardment
    Authors:  Liang-Zi Zhou and Thomas Dresselhaus, date: 12/05/2019, view: 2563, Q&A: 0
    Maize is one of the most important crop species and serves also as a model plant for grass research. A major bottleneck in maize research is stable transformation, which is both time and cost consuming, but also a technical challenge for most labs ...
    Biolistic Bombardment for Co-expression of Proteins Fused to YFP and mRFP in Leaf Epidermal Cells of Phaseolus vulgaris ‘Red Mexican’
    Author:  Malgorzata Lichocka, date: 01/05/2014, view: 11184, Q&A: 0
    Biolistic bombardment is based on coating of tungsten or gold particles with DNA and delivery of these “biobullets” into living plant cells under high pressure (Sudowe and Reske-Kunz, 2013). This method enables transient expression of a DNA ...
    Maize Embryo Transient Transformation by Particle Bombardment
    Author:  Matilde Jose-Estanyol, date: 08/20/2013, view: 7788, Q&A: 0
    Particle bombardment has been shown to be a useful method to study gene promoter regulatory elements by transient transformation of maize embryos with different constructions of gene promoters fused to a gene reporter. DNA to transfer is coated to ...



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