Molecular Biology

Categories

    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 10613, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    RNA Interference (RNAi) by Bacterial Feeding
    Author:  Fanglian He, date: 04/20/2011, view: 24838, Q&A: 0
    There are 3 ways to perform RNAi in worms: microinjection, soaking and feeding. In the feeding protocol, RNAi is induced by cultivating worms on bacteria expressing gene-specific dsRNA. dsRNA is expressed in E. coli and ingested by worms. ...
    siRNA Transfection of Mouse Bone Marrow-derived Macrophages
    Author:  Ran Chen, date: 02/05/2011, view: 21005, Q&A: 2
    Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.