Molecular Biology


    Protocol for knockdown of HuR with siRNA
    Author:  FengZhi Liu, date: 05/20/2012, view: 10613, Q&A: 1
    Through a base-pairing-dependent mechanism, 21-23 nucleotide (nt) siRNA binds to complementary target mRNA to inhibit translation.
    RNA Interference (RNAi) by Bacterial Feeding
    Author:  Fanglian He, date: 04/20/2011, view: 24838, Q&A: 0
    There are 3 ways to perform RNAi in worms: microinjection, soaking and feeding. In the feeding protocol, RNAi is induced by cultivating worms on bacteria expressing gene-specific dsRNA. dsRNA is expressed in E. coli and ingested by worms. ...
    siRNA Transfection of Mouse Bone Marrow-derived Macrophages
    Author:  Ran Chen, date: 02/05/2011, view: 21005, Q&A: 2
    Short interfering RNAs (siRNA) are a type of double-stranded RNA molecule, typically 20-25 bp long, that are involved in the phenomenon of RNA interference. siRNA transfection is employed in this protocol to knockdown target gene expression in ...

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