Molecular Biology

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    Protein Immunoprecipitation in Maize
    Author:  María Jazmín Abraham-Juárez, date: 06/05/2019, view: 1158, Q&A: 0
    Protein immunoprecipitation (IP) is a method used to identify cellular protein complexes. Whence, antibodies that specifically recognize a native bait protein or an epitope tag fused to the protein of interest allow the protein of interest to be ...
    In vitro Chaperone Activity Assay Using α-Amylase as Target Protein
    Authors:  Jeeshma Nambidi Parambath, Gayathri Valsala, Karthik Menon and Shiburaj Sugathan, date: 06/20/2018, view: 3676, Q&A: 0
    Small heat shock proteins (sHSP) are stress proteins which are ubiquitously found in almost all living organisms. They function as molecular chaperones, which assist in protein folding during translation and in the prevention of irreversible protein ...
    Rabbit IgG Conjugation to Dynabeads
    Author:  Xiyan Li, date: 05/05/2011, view: 14454, Q&A: 1
    This method couples rabbit IgG (or any other proteins serve as affinity reagent) to the surface of magnetic beads (Brand name: Dynabeads). The amine and thiol groups of amino acid residues on the protein are covalently linked with the epoxy group on ...



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