Molecular Biology

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Western Blot in Maize

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    Activation of ER-regulated Fusion Proteins In Vivo
    Author:  Jason Reuter, date: 12/20/2011, view: 9783, Q&A: 0
    Regulatable protein fusions can be made by attaching the hormone binding domain of the estrogen receptor to the N or C-terminus of a protein of interest. Activation of such ER-fusion proteins in vivo can be achieved by daily administration ...
    Infiltration of Nicotiana benthamiana Protocol for Transient Expression via Agrobacterium
    Author:  Xiyan Li, date: 07/20/2011, view: 48084, Q&A: 2
    Transient expression in tobacco plant (Nicotiana benthamiana) is used to determine the subcellular location of a protein of interest when tagged with a reporter such as green fluorescent protein (GFP), or to mass produce proteins without ...
    A Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts
    Author:  Xiyan Li, date: 05/20/2011, view: 15729, Q&A: 0
    This method can be used to free and separate the mesophyll cells from Arabidopsis leaves. The protoplasts that are generated in this way can be used for transient expression for protein activity and subcellular localization assays.