Cell Biology

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Western Blot in Maize

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    Combinations of Patch-Clamp and Confocal Calcium Imaging in Acutely Isolated Adult Mouse Amygdala Brain Slices
    Authors:  Erin E. Koffman and Jianyang Du, date: 08/05/2018, view: 2710, Q&A: 0
    Calcium imaging is a powerful technique in the study of neuronal physiology, as it avoids the enzyme treatment on neurons and is able to study the neuronal activities in vivo. Using calcium-imaging techniques, we are able to monitor the ...
    Protocol to Determine Mitotic Index by FACS
    Author:  Hui Zhu, date: 03/20/2012, view: 15240, Q&A: 0
    Fluorescence Activated Cell Sorting (FACS) is a sensitive method to count mitotic cells. Cells are stained with an antibody that recognizes an antigen present only in mitotic cells, combined with propidium iodide (PI) to stain DNA. Two-dimensional ...
    pH3 Antibody Staining Protocol for Zebrafish
    Author:  Lili Jing, date: 01/20/2012, view: 21799, Q&A: 0
    Phosphorylation of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Staining for phosphor histone H3 is a good indicator for the proliferation of the interested cells. This protocol provides a method to ...
    Western Blot for Detecting Phosphorylated STAT3
    Author:  Huagang Zhang, date: 08/20/2011, view: 20279, Q&A: 0
    The STAT3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors and is constitutively activated in a number of human tumors and possesses oncogenic potential and anti-apoptotic activities. STAT3 is ...