Molecular Biology

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Western Blot in Maize

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    Modifications in the Transformation Step of Commercially Available Site-directed Mutagenesis Kit Increase Its Price-performance
    Site-directed mutagenesis helps unravel the relationship between the structure and function of a protein at the level of amino acid contributions. Many techniques have been established to carry out site-directed mutagenesis on a daily routine. ...
    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    Authors:  Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 397, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 964, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
    Agrobacterium-mediated Transformation of Nicotiana tabacum cv. Xanthi Leaf Explants
    Authors:  Ana Lilia Rosales-Campos and Abel Gutiérrez-Ortega, date: 01/20/2019, view: 2045, Q&A: 0
    Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is Nicotiana tabacum ...
    Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
    Authors:  Wan-Qiu Wang and Wei-Hua Tang, date: 08/20/2018, view: 1919, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 3184, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...
    Root Gall Formation, Resting Spore Isolation and High Molecular Weight DNA Extraction of Plasmodiophora brassicae
    Authors:  Sara Mehrabi, Suzana Stjelja and Christina Dixelius, date: 06/05/2018, view: 2396, Q&A: 0
    Isolation of DNA from obligate biotrophic soil-borne plant pathogens is challenging. This is because of their strict requirement of living plant tissue for their growth and propagation. A soil habitat further imposes risk of contamination from other ...
    Growing and Pollinating Maize
    Authors:  Sarah Hake and China Lunde, date: 05/05/2018, view: 2143, Q&A: 0
    [Abstract] Corn, also known as maize, has been a model organism for genetics since the 1900’s due to the ease of pollinations, large ...
    Nuclear Transformation of Chlamydomonas reinhardtii by Electroporation
    Author:  Tyler M Wittkopp, date: 05/05/2018, view: 4335, Q&A: 0
    The unicellular green alga Chlamydomonas reinhardtii is an important model organism for studying photosynthesis, acclimation to abiotic stress, cilia biology, and many other biological processes. Many molecular biology tools exist for ...