Molecular Biology

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    Barley Transformation by Particle Bombardment Using Callus
    Author:  Sung-Yong Kim, date: 06/05/2020, view: 208, Q&A: 0
    The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...
    Using Seed Chipping to Genotype Maize Kernels
    Authors:  Alison M. Mills, Lindy A. Allsman, Sareen Leon and Carolyn G. Rasmussen, date: 03/20/2020, view: 568, Q&A: 0
    Zea mays (maize) is an important model organism for studying monocot growth and development. Genotyping maize in the greenhouse or field can be time consuming and costly. Here, we describe a method to remove or chip a small amount of the ...
    Different Methods of Soil DNA Extraction
    Authors:  Asmita Kamble and Harinder Singh, date: 01/20/2020, view: 1246, Q&A: 0
    Soil is the major reservoir of microbial diversity. Only 1% of microbial diversity can be cultured while 99% is still not culturable. It is necessary to extract DNA from soil in order to explore the 99% microbial diversity, which will be useful to ...
    Modifications in the Transformation Step of Commercially Available Site-directed Mutagenesis Kit Increase Its Price-performance
    Site-directed mutagenesis helps unravel the relationship between the structure and function of a protein at the level of amino acid contributions. Many techniques have been established to carry out site-directed mutagenesis on a daily routine. ...
    Fluorescence Immunolocalization in Mitotic Chromosome Spreads from Maize Embryos
    Authors:  Jing-Han Liu, Ya-zhong Wang, Lin Chen and Yan He, date: 06/20/2019, view: 1134, Q&A: 0
    Immunolocalization is a necessary technique in the study of chromosome structure and function. It can provide important information for better understanding the relation between epigenetic modifications and chromosome condensation. In this protocol, ...
    Isolation of PCR-quality Genomic DNA from Soils Impacted with Extra Heavy Crude Oil
    For the study of microbial communities in samples of soils impacted with extra heavy crude oil, it is necessary to perform molecular analyses. Due to the difficulty of oil matrix handling, there are very few protocols reported in writing. Also, one ...
    Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
    Authors:  Jarrett Man and Madelaine E. Bartlett, date: 05/05/2019, view: 2174, Q&A: 0
    CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
    Agrobacterium-mediated Transformation of Nicotiana tabacum cv. Xanthi Leaf Explants
    Authors:  Ana Lilia Rosales-Campos and Abel Gutiérrez-Ortega, date: 01/20/2019, view: 4429, Q&A: 0
    Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is Nicotiana tabacum ...
    Generation of Fusarium graminearum Knockout Mutants by the Split-marker Recombination Approach
    Authors:  Wan-Qiu Wang and Wei-Hua Tang, date: 08/20/2018, view: 2813, Q&A: 0
    Fusarium graminearum is a destructive phytopathogen and shows an impressive metabolic diversity. Gene deletion is an important and useful approach for gene function study. Here we present a protocol for generating gene deletion mutant by ...
    Plasmid Extract from Budding Yeast (Saccharomyces cerevisiae)
    Author:  Gal Haimovich, date: 07/20/2018, view: 4944, Q&A: 0
    Plasmids are widely used tools in yeast research. In many cases, plasmid libraries are used in genetic screens or in yeast two hybrid screens. In such cases, it is necessary to extract plasmids carrying unknown genetic elements from positive clones ...



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