Education

Ph.D. in Molecular Genetics, Institute of Applied Microbiology, The University of Tokyo, 1978

Current position

Professor Emeritus, Rikkyo University, Japan

Publication (since 2000)

1. Wannarat, W., Motoyama, S., Masuda, K., Kawamura, F. and Inaoka, T. (2014). Tetracycline tolerance mediated by gene amplification in Bacillus subtilis. Microbiology 160(Pt 11): 2474-2480.
   
2. Takada, H., Morita, M., Shiwa, Y., Sugimoto, R., Suzuki, S., Kawamura, F. and Yoshikawa, H. (2014). Cell motility and biofilm formation in Bacillus subtilis are affected by the ribosomal proteins, S11 and S21. Biosci Biotechnol Biochem 78(5): 898-907.
   
3. Suzuki, S., Tanigawa, O., Akanuma, G., Nanamiya, H., Kawamura, F., Tagami, K., Nomura, N., Kawabata, T. and Sekine, Y. (2014). Enhanced expression of Bacillus subtilis yaaA can restore both the growth and the sporulation defects caused by mutation of rplB, encoding ribosomal protein L2. Microbiology 160(Pt 6): 1040-1053.
   
4. Yano, K., Wada, T., Suzuki, S., Tagami, K., Matsumoto, T., Shiwa, Y., Ishige, T., Kawaguchi, Y., Masuda, K., Akanuma, G., Nanamiya, H., Niki, H., Yoshikawa, H. and Kawamura, F. (2013). Multiple rRNA operons are essential for efficient cell growth and sporulation as well as outgrowth in Bacillus subtilis. Microbiology 159(Pt 11): 2225-2236.
   
5. Akanuma, G., Suzuki, S., Yano, K., Nanamiya, H., Natori, Y., Namba, E., Watanabe, K., Tagami, K., Takeda, T., Iizuka, Y., Kobayashi, A., Ishizuka, M., Yoshikawa, H. and Kawamura, F. (2013). Single mutations introduced in the essential ribosomal proteins L3 and S10 cause a sporulation defect in Bacillus subtilis. J Gen Appl Microbiol 59(2): 105-117.
   
6. Tanaka, Y., Nanamiya, H., Yano, K., Kakugawa, K., Kawamura, F. and Ochi, K. (2013). rRNA (rrn) operon-engineered Bacillus subtilis as a feasible test organism for antibiotic discovery. Antimicrob Agents Chemother 57(4): 1948-1951.
   
7. Akanuma, G., Nanamiya, H., Natori, Y., Yano, K., Suzuki, S., Omata, S., Ishizuka, M., Sekine, Y. and Kawamura, F. (2012). Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation. J Bacteriol 194(22): 6282-6291.
   
8. Tagami, K., Nanamiya, H., Kazo, Y., Maehashi, M., Suzuki, S., Namba, E., Hoshiya, M., Hanai, R., Tozawa, Y., Morimoto, T., Ogasawara, N., Kageyama, Y., Ara, K., Ozaki, K., Yoshida, M., Kuroiwa, H., Kuroiwa, T., Ohashi, Y. and Kawamura, F. (2012). Expression of a small (p)ppGpp synthetase, YwaC, in the (p)ppGpp(0) mutant of Bacillus subtilis triggers YvyD-dependent dimerization of ribosome. Microbiologyopen 1(2): 115-134.
   
9. Shimotohno, K. W., Kawamura, F., Natori, Y., Nanamiya, H., Magae, J., Ogata, H., Endo, T., Suzuki, T. and Yamaki, H. (2010). Inhibition of septation in Bacillus subtilis by a peptide antibiotic, edeine B(1). Biol Pharm Bull 33(4): 568-571.
   
10. Nanamiya, H. and Kawamura, F. (2010). Towards an elucidation of the roles of the ribosome during different growth phases in Bacillus subtilis. Biosci Biotechnol Biochem 74(3): 451-461.
    
11. Nanamiya, H., Sato, M., Masuda, K., Sato, M., Wada, T., Suzuki, S., Natori, Y., Katano, M., Akanuma, G. and Kawamura, F. (2010). Bacillus subtilis mutants harbouring a single copy of the rRNA operon exhibit severe defects in growth and sporulation. Microbiology 156(Pt 10): 2944-2952.
    
12. Ochi, K., Kim, J. Y., Tanaka, Y., Wang, G., Masuda, K., Nanamiya, H., Okamoto, S., Tokuyama, S., Adachi, Y. and Kawamura, F. (2009). Inactivation of KsgA, a 16S rRNA methyltransferase, causes vigorous emergence of mutants with high-level kasugamycin resistance. Antimicrob Agents Chemother 53(1): 193-201.
    
13. Natori, Y., Tagami, K., Murakami, K., Yoshida, S., Tanigawa, O., Moh, Y., Masuda, K., Wada, T., Suzuki, S., Nanamiya, H., Tozawa, Y. and Kawamura, F. (2009). Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC. J Bacteriol 191(14): 4555-4561.
    
14. Nanamiya, H., Kasai, K., Nozawa, A., Yun, C. S., Narisawa, T., Murakami, K., Natori, Y., Kawamura, F. and Tozawa, Y. (2008). Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis. Mol Microbiol 67(2): 291-304.
    
15. Soma, A., Onodera, A., Sugahara, J., Kanai, A., Yachie, N., Tomita, M., Kawamura, F. and Sekine, Y. (2007). Permuted tRNA genes expressed via a circular RNA intermediate in Cyanidioschyzon merolae. Science 318(5849): 450-453.
    
16. Morohashi, M., Ohashi, Y., Tani, S., Ishii, K., Itaya, M., Nanamiya, H., Kawamura, F., Tomita, M. and Soga, T. (2007). Model-based definition of population heterogeneity and its effects on metabolism in sporulating Bacillus subtilis. J Biochem 142(2): 183-191.
    
17. Nishimura, K., Johansen, S. K., Inaoka, T., Hosaka, T., Tokuyama, S., Tahara, Y., Okamoto, S., Kawamura, F., Douthwaite, S. and Ochi, K. (2007). Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants. J Bacteriol 189(16): 6068-6073.
    
18. Asai, K., Inaoka, T., Nanamiya, H., Sadaie, Y., Ochi, K. and Kawamura, F. (2007). Isolation and characterization of sporulation-initiation mutation in the Bacillus subtilis prfB gene. Biosci Biotechnol Biochem 71(2): 397-406.
    
19. Natori, Y., Nanamiya, H., Akanuma, G., Kosono, S., Kudo, T., Ochi, K. and Kawamura, F. (2007). A fail-safe system for the ribosome under zinc-limiting conditions in Bacillus subtilis. Mol Microbiol 63(1): 294-307.
    
20. Nanamiya, H., Kawamura, F. and Kosono, S. (2006). Proteomic study of the Bacillus subtilis ribosome: Finding of zinc-dependent replacement for ribosomal protein L31 paralogues. J Gen Appl Microbiol 52(5): 249-258.
    
21. Yoshida, Y., Kuroiwa, H., Misumi, O., Nishida, K., Yagisawa, F., Fujiwara, T., Nanamiya, H., Kawamura, F. and Kuroiwa, T. (2006). Isolated chloroplast division machinery can actively constrict after stretching. Science 313(5792): 1435-1438.
    
22. Morinaga, T., Yamaguchi, M., Makino, Y., Nanamiya, H., Takahashi, K., Yoshikawa, H., Kawamura, F., Ashida, H. and Yoshida, K. (2006). Functional myo-inositol catabolic genes of Bacillus subtilis Natto are involved in depletion of pinitol in Natto (fermented soybean). Biosci Biotechnol Biochem 70(8): 1913-1920.
    
23. Koga, K., Ikegami, A., Nakasone, K., Murayama, R., Akanuma, G., Natori, Y., Nanamiya, H. and Kawamura, F. (2006). Construction of Bacillus subtilis strains carrying the transcriptional bgaB fusion with the promoter region of each rrn operon and their differential transcription during spore development. J Gen Appl Microbiol 52(2): 119-124.
    
24. Akanuma, G., Habu, C., Natori, Y., Murayama, R., Nanamiya, H. and Kawamura, F. (2006). Construction and characterization of Bacillus subtilis deletion mutants lacking the prophage 2-trnS region. FEMS Microbiol Lett 258(2): 220-226.
    
25. Akanuma, G., Nanamiya, H., Natori, Y., Nomura, N. and Kawamura, F. (2006). Liberation of zinc-containing L31 (RpmE) from ribosomes by its paralogous gene product, YtiA, in Bacillus subtilis. J Bacteriol 188(7): 2715-2720.
    
26. Matsuoka, S., Arai, T., Murayama, R., Kawamura, F., Asai, K. and Sadaie, Y. (2004). Identification of the nonA and nonB loci of Bacillus subtilis Marburg permitting the growth of SP10 phage. Genes Genet Syst 79(6): 311-317.
    
27. Murayama, R., Akanuma, G., Makino, Y., Nanamiya, H. and Kawamura, F. (2004). Spontaneous transformation and its use for genetic mapping in Bacillus subtilis. Biosci Biotechnol Biochem 68(8): 1672-1680.
    
28. Kada, S., Nanamiya, H., Kawamura, F. and Horinouchi, S. (2004). Glr, a glutamate racemase, supplies D-glutamate to both peptidoglycan synthesis and poly-gamma-glutamate production in gamma-PGA-producing Bacillus subtilis. FEMS Microbiol Lett 236(1): 13-20.
    
29. Sasaki, M., Kawamura, F. and Kurusu, Y. (2004). Genetic analysis of an incomplete bio operon in a biotin auxotrophic strain of Bacillus subtilis natto OK2. Biosci Biotechnol Biochem 68(3): 739-742.
    
30. Nanamiya, H., Akanuma, G., Natori, Y., Murayama, R., Kosono, S., Kudo, T., Kobayashi, K., Ogasawara, N., Park, S. M., Ochi, K. and Kawamura, F. (2004). Zinc is a key factor in controlling alternation of two types of L31 protein in the Bacillus subtilis ribosome. Mol Microbiol 52(1): 273-283.
    
31. Ohashi, Y., Inaoka, T., Kasai, K., Ito, Y., Okamoto, S., Satsu, H., Tozawa, Y., Kawamura, F. and Ochi, K. (2003). Expression profiling of translation-associated genes in sporulating Bacillus subtilis and consequence of sporulation by gene inactivation. Biosci Biotechnol Biochem 67(10): 2245-2253.
    
32. Nanamiya, H., Shiomi, E., Ogura, M., Tanaka, T., Asai, K. and Kawamura, F. (2003). Involvement of ClpX protein in the post-transcriptional regulation of a competence specific transcription factor, ComK protein, of Bacillus subtilis. J Biochem 133(3): 295-302.
    
33. Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., Arnaud, M., Asai, K., Ashikaga, S., Aymerich, S., Bessieres, P., Boland, F., Brignell, S. C., Bron, S., Bunai, K., Chapuis, J., Christiansen, L. C., Danchin, A., Debarbouille, M., Dervyn, E., Deuerling, E., Devine, K., Devine, S. K., Dreesen, O., Errington, J., Fillinger, S., Foster, S. J., Fujita, Y., Galizzi, A., Gardan, R., Eschevins, C., Fukushima, T., Haga, K., Harwood, C. R., Hecker, M., Hosoya, D., Hullo, M. F., Kakeshita, H., Karamata, D., Kasahara, Y., Kawamura, F., Koga, K., Koski, P., Kuwana, R., Imamura, D., Ishimaru, M., Ishikawa, S., Ishio, I., Le Coq, D., Masson, A., Mauel, C., Meima, R., Mellado, R. P., Moir, A., Moriya, S., Nagakawa, E., Nanamiya, H., Nakai, S., Nygaard, P., Ogura, M., Ohanan, T., O'Reilly, M., O'Rourke, M., Pragai, Z., Pooley, H. M., Rapoport, G., Rawlins, J. P., Rivas, L. A., Rivolta, C., Sadaie, A., Sadaie, Y., Sarvas, M., Sato, T., Saxild, H. H., Scanlan, E., Schumann, W., Seegers, J. F., Sekiguchi, J., Sekowska, A., Seror, S. J., Simon, M., Stragier, P., Studer, R., Takamatsu, H., Tanaka, T., Takeuchi, M., Thomaides, H. B., Vagner, V., van Dijl, J. M., Watabe, K., Wipat, A., Yamamoto, H., Yamamoto, M., Yamamoto, Y., Yamane, K., Yata, K., Yoshida, K., Yoshikawa, H., Zuber, U. and Ogasawara, N. (2003). Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A 100(8): 4678-4683.
    
34. Nanamiya, H., Takahashi, K., Fujita, M. and Kawamura, F. (2000). Deficiency of the initiation events of sporulation in Bacillus subtilis clpP mutant can be suppressed by a lack of the Spo0E protein phosphatase. Biochem Biophys Res Commun 279(1): 229-233.
    
35. Nanamiya, H., Fugono, N., Asai, K., Doi, R. H. and Kawamura, F. (2000). Suppression of temperature-sensitive sporulation mutation in the Bacillus subtilis sigA gene by rpoB mutation. FEMS Microbiol Lett 192(2): 237-241.
    
36. Ashikaga, S., Nanamiya, H., Ohashi, Y. and Kawamura, F. (2000). Natural genetic competence in Bacillus subtilis natto OK2. J Bacteriol 182(9): 2411-2415.
    
37. Kobayashi, H., Ohashi, Y., Nanamiya, H., Asai, K. and Kawamura, F. (2000). Genetic analysis of SecA-SecY interaction required for spore development in Bacillus subtilis. FEMS Microbiol Lett 184(2): 285-289.
    
38. Kosono, S., Ohashi, Y., Kawamura, F., Kitada, M. and Kudo, T. (2000). Function of a principal Na(+)/H(+) antiporter, ShaA, is required for initiation of sporulation in Bacillus subtilis. J Bacteriol 182(4): 898-904.