Education

Ph.D. in Science, Osaka University, Osaka, Japan

Current position

Professor, Department of Stem Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University

Publications (since 2003)

1. Yamazoe, T., Shiraki, N., Toyoda, M., Kiyokawa, N., Okita, H., Miyagawa, Y., Akutsu, H., Umezawa, A., Sasaki, Y., Kume, K. and Kume, S. (2013). A synthetic nanofibrillar matrix promotes in vitro hepatic differentiation of embryonic stem cells and induced pluripotent stem cells. J Cell Sci 126(Pt 23): 5391-5399.
   
2. Iwashita, H., Shiraki, N., Sakano, D., Ikegami, T., Shiga, M., Kume, K. and Kume, S. (2013). Secreted cerberus1 as a marker for quantification of definitive endoderm differentiation of the pluripotent stem cells. PLoS One 8(5): e64291.
   
3. Ogaki, S., Shiraki, N., Kume, K. and Kume, S. (2013). Wnt and Notch signals guide embryonic stem cell differentiation into the intestinal lineages. Stem Cells 31(6): 1086-1096.
   
4. Kataoka, M., Kawamuro, Y., Shiraki, N., Miki, R., Sakano, D., Yoshida, T., Yasukawa, T., Kume, K. and Kume, S. (2013). Recovery from diabetes in neonatal mice after a low-dose streptozotocin treatment. Biochem Biophys Res Commun 430(3): 1103-1108.
   
5. Umeda, K., Suzuki, K., Yamazoe, T., Shiraki, N., Higuchi, Y., Tokieda, K., Kume, K., Mitani, K. and Kume, S. (2013). Albumin gene targeting in human embryonic stem cells and induced pluripotent stem cells with helper-dependent adenoviral vector to monitor hepatic differentiation. Stem Cell Res 10(2): 179-194.
   
6. Ueno, T., Tomita, J., Tanimoto, H., Endo, K., Ito, K., Kume, S. and Kume, K. (2012). Identification of a dopamine pathway that regulates sleep and arousal in Drosophila. Nat Neurosci 15(11): 1516-1523.
   
7. Miki, R., Yoshida, T., Murata, K., Oki, S., Kume, K. and Kume, S. (2012). Fate maps of ventral and dorsal pancreatic progenitor cells in early somite stage mouse embryos. Mech Dev 128(11-12): 597-609.
   
8. Shiraki, N., Yamazoe, T., Qin, Z., Ohgomori, K., Mochitate, K., Kume, K. and Kume, S. (2011). Efficient differentiation of embryonic stem cells into hepatic cells in vitro using a feeder-free basement membrane substratum. PLoS One 6(8): e24228.
   
9. Katsumoto, K. and Kume, S. (2011). Endoderm and mesoderm reciprocal signaling mediated by CXCL12 and CXCR4 regulates the migration of angioblasts and establishes the pancreatic fate. Development 138(10): 1947-1955.
   
10. Ogaki, S., Harada, S., Shiraki, N., Kume, K. and Kume, S. (2011). An expression profile analysis of ES cell-derived definitive endodermal cells and Pdx1-expressing cells. BMC Dev Biol 11: 13.
    
11. Matsuo, A., Yoshida, T., Yasukawa, T., Miki, R., Kume, K. and Kume, S. (2011). Epiplakin1 is expressed in the cholangiocyte lineage cells in normal liver and adult progenitor cells in injured liver. Gene Expr Patterns 11(3-4): 255-262.
    
12. Higuchi, Y., Shiraki, N., Yamane, K., Qin, Z., Mochitate, K., Araki, K., Senokuchi, T., Yamagata, K., Hara, M., Kume, K. and Kume, S. (2010). Synthesized basement membranes direct the differentiation of mouse embryonic stem cells into pancreatic lineages. J Cell Sci 123(Pt 16): 2733-2742.
    
13. Shiraki, N., Harada, S., Ogaki, S., Kume, K. and Kume, S. (2010). Identification of DAF1/CD55, a novel definitive endoderm marker. Cell Struct Funct 35(2): 73-80.
    
14. Matsuura, K., Katsumoto, K., Fukuda, K., Kume, K. and Kume, S. (2009). Conserved origin of the ventral pancreas in chicken. Mech Dev 126(10): 817-827.
    
15. Katsumoto, K., Fukuda, K., Kimura, W., Shimamura, K., Yasugi, S. and Kume, S. (2009). Origin of pancreatic precursors in the chick embryo and the mechanism of endoderm regionalization. Mech Dev 126(7): 539-551.
    
16. Yoshida, T., Murata, K., Shiraki, N., Kume, K. and Kume, S. (2009). Analysis of gene expressions of embryonic stem-derived Pdx1-expressing cells: implications of genes involved in pancreas differentiation. Dev Growth Differ 51(4): 463-472.
    
17. Shiraki, N., Higuchi, Y., Harada, S., Umeda, K., Isagawa, T., Aburatani, H., Kume, K. and Kume, S. (2009). Differentiation and characterization of embryonic stem cells into three germ layers. Biochem Biophys Res Commun 381(4): 694-699.
    
18. Shiraki, N., Umeda, K., Sakashita, N., Takeya, M., Kume, K. and Kume, S. (2008). Differentiation of mouse and human embryonic stem cells into hepatic lineages. Genes Cells 13(7): 731-746.
    
19. Yoshida, T., Shiraki, N., Baba, H., Goto, M., Fujiwara, S., Kume, K. and Kume, S. (2008). Expression patterns of epiplakin1 in pancreas, pancreatic cancer and regenerating pancreas. Genes Cells 13(7): 667-678.
    
20. Shiraki, N., Yoshida, T., Araki, K., Umezawa, A., Higuchi, Y., Goto, H., Kume, K. and Kume, S. (2008). Guided differentiation of embryonic stem cells into Pdx1-expressing regional-specific definitive endoderm. Stem Cells 26(4): 874-885.
    
21. Shiraki, N., Lai, C. J., Hishikari, Y. and Kume, S. (2005). TGF-beta signaling potentiates differentiation of embryonic stem cells to Pdx-1 expressing endodermal cells. Genes Cells 10(6): 503-516.
    
22. Rajagopal, J., Anderson, W. J., Kume, S., Martinez, O. I. and Melton, D. A. (2003). Insulin staining of ES cell progeny from insulin uptake. Science 299(5605): 363.