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A peer-reviewed protocol journal. No publication fee; no access fee.
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Coauthors
Clara Sciorati
Division of Regenerative Medicine, Ospedale San Raffaele, Italy
1 protocol
Laura Pozzi
Scientific Institute, Italy
1 protocol
Viviana Pisa
Department of Biomedical and Clinical Sciences, University of Milano, Italy
1 protocol
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Coreviewers
Abhijit Kale
Buck Institute for Research on Aging
2 protocols
Agnieszka Pastula
Columbia University in the City of New York
4 protocols
Alexandros Alexandratos
University of Athens
10 protocols
Antoine de Morree
Stanford university
32 protocols
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Editor
Nicoletta Cordani
Post-Doc, University of Milano-Bicocca
Research focus
Cancer biology
Contributions
1
Author merit
Reviewer merit
Editor merit
Lab information
University of Milano-Bicocca
Publications
https://www.ncbi.nlm.nih.gov/pubmed/?term=cordani+n
1 Protocol published
Isolation of FAP Cells from Mouse Dystrophic Skeletal Muscle Using Fluorescence Activated Cell Sorting
Authors:
Nicoletta Cordani
,
Viviana Pisa
,
Laura Pozzi
and
Clara Sciorati
,
date:
11/20/2014,
view:
6053,
Q&A:
1
A population of muscle resident CD45
-
, CD31
-
cells expressing the mesenchymal PDGF receptor alpha (PDGFRα) as well as Sca-1 was first isolated in healthy mouse muscles in Uezumi
et al.
(2010). In the same year, Joe
et
...
More >
Huan Li
Sun Yat-sen University
I want to know the size of FAP cells since the information is important for my experiment. I am looking forward to the answer. Thank you!
5/11/2018 9:43:50 PM
Reply
Huan Li
Sun Yat-sen University
Thank you for your answer! It is helpful.
2018-05-27 23:46:28
Clara Sciorati
Division of Regenerative Medicine, Ospedale San Raffaele, Italy
Hi
what do you mean for size? they are quite similar to satellite cells...
2018-05-27 23:39:16
Clara Sciorati
Division of Regenerative Medicine, Ospedale San Raffaele, Italy
In plate FAP cells are around 20-25um.
All my best and cross the finger for your experiment!
2018-05-27 12:25:52
3 Protocols reviewed
Differentiation of Human Embryonic Stem Cells into Cone Photoreceptors
Authors:
Anthony Flamier
,
Andrea Barabino
and
Gilbert Bernier
,
date:
07/20/2016,
view:
3508,
Q&A:
0
Photoreceptors are specialized retinal neurons able to respond to light in order to generate visual information. Among photoreceptors, cones are involved in colors discrimination and high-resolution central vision and are selectively depleted in ...
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Laser Microirradiation and Temporal Analysis of XRCC1 Recruitment to Single-strand DNA Breaks
Authors:
Anna Campalans
and
J. Pablo Radicella
,
date:
03/05/2016,
view:
3797,
Q&A:
0
The DNA molecule is exposed to a multitude of damaging agents that can compromise its integrity: single (SSB) and double strand breaks (DSB), intra- or inter-strand crosslinks, base loss or modification,
etc.
Many different DNA repair ...
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17 Protocols edited
Qualitative
in vivo
Bioluminescence Imaging
Authors:
Devbarna Sinha
,
Zalitha Pieterse
and
Pritinder Kaur
,
date:
09/20/2018,
view:
79,
Q&A:
0
Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals
in vivo
. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ...
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Osteoblast Sorting and Intracellular Staining of CXCL12
Authors:
Weihuan Wang
,
Gurnoor Majhail
,
Cui Lui
and
Lan Zhou
,
date:
05/20/2018,
view:
834,
Q&A:
0
Osteoblasts are bone marrow endosteum-lining niche cells playing important roles in the regulation of hematopoietic stem cells by secreting factors and cell adhesion molecules. Characterization of primary osteoblasts has been achieved through ...
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