Andrea Puhar —BIO-PROTOCOL

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Coauthors

Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols

Coreviewers

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos University of Edinburgh
3 protocols

Ana Santos Almeida University College Cork
4 protocols

Bin Tian Kansas State University
5 protocols

Editor

Andrea Puhar

Faculty, MIMS - Umea University

Research focus

Immunology
Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Gut

2 Author merit
Reviewer merit
Editor merit

Education

Ph.D., University of Padova, Italy

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

https://scholar.google.se/citations?user=ok2BcpoAAAAJ&hl=en&oi=ao

2 Protocols published

Dye-uptake Experiment through Connexin Hemichannels

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 5485, Q&A: 0

Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...

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Induction of Connexin-hemichannel Opening

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 3759, Q&A: 0

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6 Protocols reviewed

Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting

Authors: Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson, date: 02/05/2018, view: 657, Q&A: 0

To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...

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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles

Authors: Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 2462, Q&A: 0

In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...

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23 Protocols edited

Immunohistochemical Identification of Human Skeletal Muscle Macrophages

Authors: Kate Kosmac, Bailey D. Peck, R. Grace Walton, Jyothi Mula, Philip A. Kern, Marcas M. Bamman, Richard A. Dennis, Cale A. Jacobs, Christian Lattermann, Darren L. Johnson and Charlotte A. Peterson, date: 06/20/2018, view: 78, Q&A: 0

Macrophages have well-characterized roles in skeletal muscle repair and regeneration. Relatively little is known regarding the role of resident macrophages in skeletal muscle homeostasis, extracellular matrix remodeling, growth, metabolism and ...

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Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation

Authors: Emilie Viennois, Anika Tahsin and Didier Merlin, date: 05/05/2018, view: 398, Q&A: 0

We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium ...

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