Andrea Puhar —BIO-PROTOCOL

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Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols

Aksiniya Asenova University of California, Davis
12 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos University of Edinburgh
4 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor

Andrea Puhar

Faculty, MIMS - Umeå University

Research focus

Immunology
Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Gut

2 Author merit
Reviewer merit
Editor merit

Lab Protocol Hub

Puhar Lab

Education

Ph.D., University of Padova, Italy

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

https://scholar.google.se/citations?user=ok2BcpoAAAAJ&hl=en&oi=ao

2 Protocols published

Dye-uptake Experiment through Connexin Hemichannels

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 6327, Q&A: 0

Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...

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Induction of Connexin-hemichannel Opening

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 4429, Q&A: 0

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7 Protocols reviewed

Correction: Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD⁺) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry

Authors: Karina S. Kanamori, Guilherme C. de Oliveira, Maria Auxiliadora-Martins, Renee A. Schoon, Joel M. Reid and Eduardo N. Chini, date: 09/05/2018, view: 174, Q&A: 0

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Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting

Authors: Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson, date: 02/05/2018, view: 1446, Q&A: 0

To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...

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33 Protocols edited

Purification of Globular Actin from Rabbit Muscle and Pyrene Fluorescent Assays to Investigate Actin Dynamics in vitro

Authors: He Sun, Yuanyuan Luo and Yansong Miao, date: 12/05/2018, view: 62, Q&A: 0

Pyrene Fluorescent Assay is established to monitor the dynamic actin nucleation, elongation, capping and disassembly in vitro. This technique provides an easy handle procedure and straightforward visual data analysis. By coupling actin ...

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Extraction and Analysis of Bacterial Teichoic Acids

Authors: Kelvin Kho and Timothy C. Meredith, date: 11/05/2018, view: 257, Q&A: 0

Teichoic acids (TA) are anionic polymers comprised of polyol phosphate repeat units that are abundant in the cell wall of Gram-positive bacteria. Both wall teichoic acid (WTA) and lipoteichoic acid (LTA) play important roles in regulating cell wall ...

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