Andrea Puhar —BIO-PROTOCOL

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Coauthors

Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols

Coreviewers

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos University of Edinburgh
4 protocols

Ana Santos Almeida University College Cork
4 protocols

Anca Savulescu University of Cape Town
13 protocols

Editor

Andrea Puhar

Faculty, MIMS - Umeå University

Research focus

Immunology
Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Gut

2 Author merit
Reviewer merit
Editor merit

Lab Protocol Hub

Puhar Lab

Education

Ph.D., University of Padova, Italy

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

https://scholar.google.se/citations?user=ok2BcpoAAAAJ&hl=en&oi=ao

2 Protocols published

Dye-uptake Experiment through Connexin Hemichannels

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 6080, Q&A: 0

Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...

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Induction of Connexin-hemichannel Opening

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 4209, Q&A: 0

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7 Protocols reviewed

Correction: Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD⁺) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry

Authors: Karina S. Kanamori, Guilherme C. de Oliveira, Maria Auxiliadora-Martins, Renee A. Schoon, Joel M. Reid and Eduardo N. Chini, date: 09/05/2018, view: 81, Q&A: 0

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Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting

Authors: Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson, date: 02/05/2018, view: 1266, Q&A: 0

To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...

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29 Protocols edited

A Highly Sensitive Anion Exchange Chromatography Method for Measuring cGAS Activity in vitro

Authors: Andreas Holleufer and Rune Hartmann, date: 10/20/2018, view: 23, Q&A: 0

Cyclic GMP-AMP synthase (cGAS) is a pattern recognition receptor (PRR) that senses double stranded DNA (dsDNA) in the cytosol and this leads to the activation of stimulator of interferon genes (STING) via the secondary messenger 2’3’-cyclic GMP-AMP ...

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Platelet Migration and Bacterial Trapping Assay under Flow

Authors: Shuxia Fan, Michael Lorenz, Steffen Massberg and Florian Gaertner, date: 09/20/2018, view: 321, Q&A: 0

Blood platelets are critical for hemostasis and thrombosis, but also play diverse roles during immune responses. We have recently reported that platelets migrate at sites of infection in vitro and in vivo. Importantly, platelets ...

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