Andrea Puhar —BIO-PROTOCOL

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Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols

Aksiniya Asenova University of California, Davis
12 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos Weill Cornell Medicine
7 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor

Andrea Puhar

Faculty, MIMS - Umeå University

Research focus

Immunology
Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut

2 Author merit
Reviewer merit
Editor merit

Lab Protocol Hub

Puhar Lab

Education

Ph.D., University of Padova, Italy, 2007

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

https://scholar.google.se/citations?user=ok2BcpoAAAAJ&hl=en&oi=ao

2 Protocols published

Dye-uptake Experiment through Connexin Hemichannels

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 7299, Q&A: 0

Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...

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Induction of Connexin-hemichannel Opening

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 5191, Q&A: 0

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6 Protocols reviewed

Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting

Authors: Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson, date: 02/05/2018, view: 2320, Q&A: 0

To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...

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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles

Authors: Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 5424, Q&A: 1

In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...

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35 Protocols edited

Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells

Authors: Nicholas Mikolajewicz and Svetlana V. Komarova, date: 05/20/2019, view: 446, Q&A: 0

Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous ...

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Correction: Measuring CD38 Hydrolase and Cyclase Activities: 1,N6-Ethenonicotinamide Adenine Dinucleotide (ε-NAD) and Nicotinamide Guanine Dinucleotide (NGD) Fluorescence-based Methods

Authors: Guilherme C. de Oliveira, Karina S. Kanamori, Maria Auxiliadora-Martins, Claudia C. S. Chini and Eduardo N. Chini, date: 05/05/2019, view: 64, Q&A: 0

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