Andrea Puhar —BIO-PROTOCOL

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Atin Sharma The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University
2 protocols

Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols

Aksiniya Asenova University of California, Davis
12 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos Weill Cornell Medicine
7 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor

Andrea Puhar

Faculty, The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden

Research focus

Immunology
Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut

4 Author merit
Reviewer merit
Editor merit

Lab Protocol Hub

Puhar Lab

Education

Ph.D., University of Padova, Italy, 2007

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

https://scholar.google.se/citations?user=ok2BcpoAAAAJ&hl=en&oi=ao

4 Protocols published

Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells

Authors: Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 216, Q&A: 0

Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...

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Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Authors: Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 252, Q&A: 0

Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified ...

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6 Protocols reviewed

Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting

Authors: Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson, date: 02/05/2018, view: 2543, Q&A: 0

To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...

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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles

Authors: Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 5784, Q&A: 1

In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...

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37 Protocols edited

Adhesive and Cohesive Peel Force Measurement of Human Airway Mucus

Authors: Henry P. Goodell, Siddharth K. Shenoy, Nathan T. Shenkute, Elijah Lackey, Robert G. Dennis and Brian Button, date: 07/05/2019, view: 316, Q&A: 0

In health, the high-speed airflow associated with cough represents a vital backup mechanism for clearing accumulated mucus from our airways. However, alterations in the mucus layer in cystic fibrosis (CF) and chronic obstructive pulmonary disease ...

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Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide

Authors: Anthony Yammine, Jinlong Gao and Ann H. Kwan, date: 06/05/2019, view: 715, Q&A: 0

Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...

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