Andrea Puhar —BIO-PROTOCOL

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Coauthors

Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols

Coreviewers

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos University of Edinburgh
3 protocols

Ana Santos Almeida University College Cork
4 protocols

Anca Savulescu University of Cape Town
13 protocols

Editor

Andrea Puhar

Faculty, MIMS - Umea University

Research focus

Immunology
Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Gut

2 Author merit
Reviewer merit
Editor merit

Education

Ph.D., University of Padova, Italy

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

https://scholar.google.se/citations?user=ok2BcpoAAAAJ&hl=en&oi=ao

2 Protocols published

Dye-uptake Experiment through Connexin Hemichannels

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 5799, Q&A: 0

Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...

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Induction of Connexin-hemichannel Opening

Authors: Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 3979, Q&A: 0

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6 Protocols reviewed

Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting

Authors: Sara Sigurlásdóttir, Sunil D Saroj, Olaspers Sara Eriksson, Jens Eriksson and Ann-Beth Jonsson, date: 02/05/2018, view: 1020, Q&A: 0

To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...

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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles

Authors: Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 2989, Q&A: 0

In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...

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27 Protocols edited

Measuring CD38 Hydrolase and Cyclase Activities: 1,N⁶-Ethenonicotinamide Adenine Dinucleotide (ε-NAD) and Nicotinamide Guanine Dinucleotide (NGD) Fluorescence-based Methods

Authors: Guilherme C. de Oliveira, Karina S. Kanamori, Maria Auxiliadora-Martins, Claudia C. S. Chini and Eduardo N. Chini, date: 07/20/2018, view: 279, Q&A: 0

CD38 is a multifunctional enzyme involved in calcium signaling and Nicotinamide Adenine Dinucleotide (NAD⁺) metabolism. Through its major activity, the hydrolysis of NAD⁺, CD38 helps maintain the appropriate levels of this ...

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Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD⁺) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry

Authors: Karina S. Kanamori, Guilherme C. de Oliveira, Maria Auxiliadora-Martins, Renee A. Schoon, Joel M. Reid and Eduardo N. Chini, date: 07/20/2018, view: 240, Q&A: 0

Current studies on the age-related development of metabolic dysfunction and frailty are each day in more evidence. It is known, as aging progresses, nicotinamide adenine dinucleotide (NAD⁺) levels decrease in an expected physiological ...

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