Atin Sharma The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University
2 protocols

Philippe Sansonetti Institut Pasteur, Unité de Pathogénie Microbienne Moléculaire, France
4 protocols
Aksiniya Asenova University of California, Davis
13 protocols

Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Alexandros Kokotos Weill Cornell Medicine
7 protocols

Ana Santos Almeida University College Cork
4 protocols

Editor
Andrea Puhar
  • Faculty, The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
Research focus
  • Immunology
  • Infection; Inflammation; Endogenous danger signals; Extracellular ATP; Intestinal bacteria; Pathogens; Microbiota; Gut
  • 4 Author merit
Lab Protocol Hub

Education

Ph.D., University of Padova, Italy, 2007

Lab information

Andrea Puhar Lab
http://www.mims.umu.se/groups/andrea-puhar.html

Publications

4 Protocols published
Plaque Assay to Determine Invasion and Intercellular Dissemination of Shigella flexneri in TC7 Human Intestinal Epithelial Cells
Authors:  Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 425, Q&A: 0
Shigella flexneri invades the epithelial cells lining the gut lumen and replicates intracellularly. The specialized Type III Secretion System (T3SS) and its effector proteins, encoded on a large virulence plasmid, assist the bacterium to ...
Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri
Authors:  Atin Sharma and Andrea Puhar, date: 07/05/2019, view: 502, Q&A: 0
Shigella flexneri is an intracellular bacterial pathogen that gains access to the gut epithelium using a specialized Type III Secretion System (T3SS). Various determinants mediating this invasive infection have been experimentally verified ...
6 Protocols reviewed
Quantification of Neisseria meningitidis Adherence to Human Epithelial Cells by Colony Counting
To cause an infection, the human specific pathogen Neisseria meningitides must first colonize the nasopharynx. Upon tight interaction with the mucosal epithelium, N. meningitidis may cross the epithelial cellular barrier, reach the ...
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Cytosolic and Nuclear Delivery of CRISPR/Cas9-ribonucleoprotein for Gene Editing Using Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 10/20/2017, view: 5942, Q&A: 1
In this protocol, engineered Cas9-ribonucleoprotein (Cas9 protein and sgRNA, together called Cas9-RNP) and gold nanoparticles are used to make nanoassemblies that are employed to deliver Cas9-RNP into cell cytoplasm and nucleus. Cas9 protein is ...
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38 Protocols edited
Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for in situ Labeling of Peptidoglycan
Authors:  Katharina Peters, Manuel Pazos, Michael S. VanNieuwenhze and Waldemar Vollmer, date: 08/05/2019, view: 181, Q&A: 0
The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves ...
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Adhesive and Cohesive Peel Force Measurement of Human Airway Mucus
In health, the high-speed airflow associated with cough represents a vital backup mechanism for clearing accumulated mucus from our airways. However, alterations in the mucus layer in cystic fibrosis (CF) and chronic obstructive pulmonary disease ...
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