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Rajneesh Laboratory of Photobiology and Molecular Microbiology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University
2 protocols

Jianguo Ruan Bio-protocol
1 protocol

hypo 单海波
  • 1 Author merit
1 Protocol published
水稻组织总RNA抽提
Authors:  hypo 单海波, Jianguo Ruan and Rajneesh, date: 08/30/2017, view: 1908, Q&A: 46
TRIzol中苯酚和硫氰酸胍,从组织细胞中快速抽提总RNA,并在匀浆和裂解细胞时保持RNA的完整性。氯仿萃取有机相后,RNA溶于水中相中,用异丙醇沉淀RNA。