Nahum Sonenberg Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Canada
1 protocol

Ivan Topisirovic Department of Oncology and Lady Davis Institute for Medical Research, Sir Mortimer B. Davis-Jewish General Hospital, McGill University, Canada
1 protocol

Tommy Alain Department of Biochemistry and Goodman Cancer Research Centre, McGill University, Canada
1 protocol
Ana Perez-Villalba Universidad de Valencia
2 protocols

Antoine de Morree Stanford university
36 protocols

Arsalan Daudi University of California
157 protocols

Daniel Kraus Mainz University Hospital
6 protocols

Masahiro Morita
  • Faculty, University of Texas Health Science Center at San Antonio
Research focus
  • Cancer biology
  • 1 Author merit
1 Protocol published
Polysome Profiling Analysis
Authors:  Masahiro Morita, Tommy Alain, Ivan Topisirovic and Nahum Sonenberg, date: 07/20/2013, view: 41947, Q&A: 2
Polysome profiling is a method that allows monitoring of translation activity of mRNAs in cells and tissues. Once each polysome fractions are collected, the translation activity of each mRNA is analyzed using various molecular biology techniques ...
13 Protocols reviewed
Analysis of N-acetylmuramic acid-6-phosphate (MurNAc-6P) Accumulation by HPLC-MS
Authors:  Marina Borisova and Christoph Mayer, date: 08/05/2017, view: 6348, Q&A: 0
We describe here in detail a high-performance liquid chromatography-mass spectrometry (HPLC-MS)-based method to determine N-acetylmuramic acid-6-phosphate (MurNAc-6P) in bacterial cell extracts. The method can be applied to both ...
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Measuring Behavioral Individuality in the Acoustic Startle Behavior in Zebrafish
Authors:  Carlos Pantoja, Adam Hoagland, Elizabeth Carroll, David Schoppik and Ehud Y. Isacoff, date: 04/05/2017, view: 7326, Q&A: 0
The objective of this protocol is to provide a detailed description for the construction and use of a behavioral apparatus, the zBox, for high-throughput behavioral measurements in larval zebrafish (Danio rerio). The zBox is used to measure ...
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14 Protocols edited
Fat Turnover Assay in Drosophila
Authors:  Subhash D. Katewa and Pankaj Kapahi, date: 11/05/2016, view: 9399, Q&A: 0
Like all animals, Drosophila shows robust fat (triglyceride) turnover, i.e., they synthesize, store and utilize triglyceride for their daily metabolic needs. The protocol describes a simple assay to measure this turnover of ...
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Generation of Mitochondrial-nuclear eXchange Mice via Pronuclear Transfer
The mitochondrial paradigm for common disease proposes that mitochondrial DNA (mtDNA) sequence variation can contribute to disease susceptibility and progression. To test this concept, we developed the Mitochondrial-nuclear eXchange (MNX) model, in ...
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