Imayavaramban Lakshmanan

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Surinder K. Batra DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, University of Nebraska Medical Center, United States
2 protocols

Reviewer

Imayavaramban Lakshmanan

Nebraska Medical Center

Research focus

Molecular biology

1 Author merit
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1 Protocol published

Protocol for Apoptosis Assay by Flow Cytometry Using Annexin V Staining Method

Authors: Imayavaramban Lakshmanan and Surinder K Batra, date: 03/20/2013, view: 19893, Q&A: 1

This assay is used to count the number of cells that have undergone apoptosis. Apoptosis will be detected by initially staining the cells with Annexin V and propidium iodide solution followed by flow cytometry analysis. It is based on the principle ...

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Eray Sahin

Yeditepe University

Hi, I'm using this Annexin-V-FLUOS staining kit and according to the protocol prepared by Roche, 100 ul of mixture(incubation buffer+annexin-V+PI) should be mixed with 1 million of cells. Does this number represent the initial amount of cells that have been seeded or for the ones that would be harvested?
Second question; how will the percentages be affected if I use less amount of staining mixture?

Thank you.

4/14/2013 4:10:53 PM Reply

Imayavaramban Lakshmanan

Nebraska Medical Center

Does this number represent the initial amount of cells that have been seeded or for the ones that would be harvested?

Yes. It represents the initial amount of cells that have been seeded

Second question; how will the percentages be affected if I use less amount of staining mixture?

For us it has worked very well for the mentioned concentration, if you want to use less amount of staining solution, we would recommend seeding less number of cells.

2013-04-15 15:53:35