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A peer-reviewed protocol journal. No publication fee; no access fee.
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Coreviewers
Antoine de Morree
Stanford university
32 protocols
Fanglian He
Bio-protocol
205 protocols
Lin Fang
Stanford University
95 protocols
Nicoletta Cordani
University of Milano-Bicocca
20 protocols
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Editor
Hui Zhu
Stanford University
Research focus
Cell biology
Contributions
9
Author merit
Reviewer merit
Editor merit
9 Protocols published
Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
Author:
Hui Zhu
,
date:
04/05/2012,
view:
16255,
Q&A:
3
Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
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Wu Sian
06-2757575
Can I use GFP-antibody to improve green florescence and then use PI stain to detect cell cycle? Any protocol can I follow?
9/7/2017 5:55:43 PM
Reply
Hui Zhu
Stanford University
I have used GFP antibody in Immunofluorescence staining. For FACS, I have never tried. If GFP+ % is fairly high, you don't need to add antibody. If GFP+ % is very low, adding antibody may not help.
2017-09-07 19:07:44
Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
Author:
Hui Zhu
,
date:
04/05/2012,
view:
48474,
Q&A:
2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
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Hello,
I hope you are well. My name Nawroz and I am PhD student at Keele University. I am wondering if I can use the brdu for CLC cell line and could you please send me more information about this method for the cell line because I want to use it to determined cell proliferation.
I appreciate your help.
Kindest regards
Nawroz O. Kareem
10/19/2012 5:27:19 AM
Reply
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17 Protocols reviewed
Isolation of Primary Human Skeletal Muscle Cells
Authors:
Janelle M. Spinazzola
and
Emanuela Gussoni
,
date:
11/05/2017,
view:
1866,
Q&A:
0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic ...
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Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from
Mactra chinensis
Authors:
Kyohei Higashi
and
Toshihiko Toida
,
date:
08/05/2017,
view:
1917,
Q&A:
0
Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), heparin (HP), heparan sulfate (HS) and keratan sulfate (KS) are linear, sulfated repeating disaccharide sequences containing hexosamine and uronic acid (or ...
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