Antoine de Morree Stanford university
32 protocols

Fanglian He Bio-protocol
205 protocols

Lin Fang Stanford University
95 protocols

Nicoletta Cordani University of Milano-Bicocca
20 protocols

Editor
Hui Zhu
  • Stanford University
Research focus
  • Cell biology
  • 9 Author merit
Lab Protocol Hub
9 Protocols published
Cell Cycle Analysis Using Propidium Iodide Staining with GFP Detection
Author:  Hui Zhu, date: 04/05/2012, view: 16784, Q&A: 3
Infecting mammalian cells with a GFP construct to overexpress or knockdown target genes is one of the most commonly used methods to study and manipulate gene expression. To determine the target gene function on the cell cycle, analyzing the cell ...
Cell Proliferation Assay by Flow Cytometry (BrdU and PI Staining)
Author:  Hui Zhu, date: 04/05/2012, view: 49607, Q&A: 2
Cell Proliferation assays include an important set of fluorescence-based tests that can monitor cell health and cell division by evaluating DNA synthesis through thymidine incorporation. Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a ...
17 Protocols reviewed
Isolation of Primary Human Skeletal Muscle Cells
Authors:  Janelle M. Spinazzola and Emanuela Gussoni, date: 11/05/2017, view: 2167, Q&A: 0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic ...
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Isolation of Keratan Sulfate Disaccharide-branched Chondroitin Sulfate E from Mactra chinensis
Authors:  Kyohei Higashi and Toshihiko Toida, date: 08/05/2017, view: 2184, Q&A: 0
Glycosaminoglycans (GAGs) including chondroitin sulfate (CS), dermatan sulfate (DS), heparin (HP), heparan sulfate (HS) and keratan sulfate (KS) are linear, sulfated repeating disaccharide sequences containing hexosamine and uronic acid (or ...
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