Feng Yue Department of Animal Science, Purdue University, USA
1 protocol

Chao Wang Purdue University
1 protocol

Beatriz Castro Purdue University
1 protocol

Timothy Gavin Department of Health and Kinesiology and Max E. Wastl Human Performance Laboratory, Purdue University, USA
1 protocol

Shihuan Kuang
  • Department of Animal Science, Purdue University, USA
  • 3 Author merit


Ph.D in Physiology and Cell Biology, Department of Biological Sciences, University of Alberta, Edmonton, Canada, 2002

Current position

Associate Professor, Department of Animal Sciences, Department of Health & Kinesiology, Center for Cancer Research, Purdue University


  1. Shan, T., Zhang, P., Bi, P. and Kuang, S. (2015). Lkb1 deletion promotes ectopic lipid accumulation in muscle progenitor cells and mature muscles. J Cell Physiol 230(5): 1033-1041.
  2. Wang, J. H., Wang, Q. J., Wang, C., Reinholt, B., Grant, A. L., Gerrard, D. E. and Kuang, S. (2015). Heterogeneous activation of a slow myosin gene in proliferating myoblasts and differentiated single myofibers. Dev Biol 402(1): 72-80.
  3. Bi, P. and Kuang, S. (2015). Notch signaling as a novel regulator of metabolism. Trends Endocrinol Metab 26(5): 248-255.
  4. Yu, H., Waddell, J. N., Kuang, S. and Bidwell, C. A. (2014). Park7 expression influences myotube size and myosin expression in muscle. PLoS One 9(3): e92030.
  5. Jiang, C., Wen, Y., Kuroda, K., Hannon, K., Rudnicki, M. A. and Kuang, S. (2014). Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy. Dis Model Mech 7(8): 997-1004.
  6. Shan, T., Zhang, P., Liang, X., Bi, P., Yue, F. and Kuang, S. (2014). Lkb1 is indispensable for skeletal muscle development, regeneration, and satellite cell homeostasis. Stem Cells 32(11): 2893-2907.
  7. Zhang, P., Shan, T., Liang, X., Deng, C. and Kuang, S. (2014). Mammalian target of rapamycin is essential for cardiomyocyte survival and heart development in mice. Biochem Biophys Res Commun 452(1): 53-59.
  8. Bi, P., Shan, T., Yang, X., Liu, W., Yue, F., Liang, X., Wang, J., Li, J,, Carlesso. N,, Liu. X. and Kuang, S. (2014). Notch signaling regulates adipose browning and energy metabolism. Nat Med 20(8): 911-8.
  9. Shan, T., Liu, W. and Kuang, S. (2013). Fatty acid binding protein 4 expression marks a population of adipocyte progenitors in white and brown adipose tissues. FASEB J 27(1): 277-287.
  10. Shan, T., Liang, X., Bi, P. and Kuang, S. (2013). Myostatin knockout drives browning of white adipose tissue through activating the AMPK-PGC1alpha-Fndc5 pathway in muscle. FASEB J 27(5): 1981-1989.
  11. Kuroda, K., Kuang, S., Taketo, M. M. and Rudnicki, M. A. (2013). Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts. Skelet Muscle 3(1): 5.
  12. Liu, W., Bi, P., Shan, T., Yang, X., Yin, H., Wang, Y. X., Liu, N., Rudnicki, M. A. and Kuang, S. (2013). miR-133a regulates adipocyte browning in vivo. PLoS Genet 9(7): e1003626.
  13. Shan, T., Liang, X., Bi, P., Zhang, P., Liu, W. and Kuang, S. (2013). Distinct populations of adipogenic and myogenic Myf5-lineage progenitors in white adipose tissues. J Lipid Res 54(8): 2214-2224.
  14. Liu, W., Shan, T., Yang, X., Liang, S., Zhang, P., Liu, Y., Liu, X. and Kuang, S. (2013). A heterogeneous lineage origin underlies the phenotypic and molecular differences of white and beige adipocytes. J Cell Sci 126(Pt 16): 3527-3532.
  15. Ogura, Y., Mishra, V., Hindi, S. M., Kuang, S. and Kumar, A. (2013). Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-kappaB signaling pathways. J Biol Chem 288(49): 35159-35169.
  16. Chakkalakal, J. V., Kuang, S., Buffelli, M., Lichtman, J. W. and Sanes, J. R. (2012). Mouse transgenic lines that selectively label Type I, Type IIA, and Types IIX+B skeletal muscle fibers. Genesis 50(1): 50-58.
  17. Wen, Y., Bi, P., Liu, W., Asakura, A., Keller, C. and Kuang, S. (2012). Constitutive Notch activation upregulates Pax7 and promotes the self-renewal of skeletal muscle satellite cells. Mol Cell Biol 32(12): 2300-2311.
  18. Liu, W., Liu, Y., Lai, X. and Kuang, S. (2012). Intramuscular adipose is derived from a non-Pax3 lineage and required for efficient regeneration of skeletal muscles. Dev Biol 361(1): 27-38.
  19. Liu, W., Wen, Y., Bi, P., Lai, X., Liu, X. S., Liu, X. and Kuang, S. (2012). Hypoxia promotes satellite cell self-renewal and enhances the efficiency of myoblast transplantation. Development 139(16): 2857-2865.
  20. Hindi, S. M., Paul, P. K., Dahiya, S., Mishra, V., Bhatnagar, S., Kuang, S., Choi, Y. and Kumar, A. (2012). Reciprocal interaction between TRAF6 and notch signaling regulates adult myofiber regeneration upon injury. Mol Cell Biol 32(23): 4833-4845.
  21. Dahiya, S., Bhatnagar, S., Hindi, S. M., Jiang, C., Paul, P. K., Kuang, S. and Kumar, A. (2011). Elevated levels of active matrix metalloproteinase-9 cause hypertrophy in skeletal muscle of normal and dystrophin-deficient mdx mice. Hum Mol Genet 20(22): 4345-4359.
  22. Angione, A. R., Jiang, C., Pan, D., Wang, Y. X. and Kuang, S. (2011). PPARdelta regulates satellite cell proliferation and skeletal muscle regeneration. Skelet Muscle 1(1): 33.
  23. Mittal, A., Bhatnagar, S., Kumar, A., Paul, P. K., Kuang, S. and Kumar, A. (2010). Genetic ablation of TWEAK augments regeneration and post-injury growth of skeletal muscle in mice. Am J Pathol 177(4): 1732-1742.
  24. Waddell, J. N., Zhang, P., Wen, Y., Gupta, S. K., Yevtodiyenko, A., Schmidt, J. V., Bidwell, C. A., Kumar, A. and Kuang, S. (2010). Dlk1 is necessary for proper skeletal muscle development and regeneration. PLoS One 5(11): e15055.
  25. Gillespie, M. A., Le Grand, F., Scime, A., Kuang, S., von Maltzahn, J., Seale, V., Cuenda, A., Ranish, J. A. and Rudnicki, M. A. (2009). p38-{gamma}-dependent gene silencing restricts entry into the myogenic differentiation program. J Cell Biol 187(7): 991-1005.
  26. Kuang, S. and Rudnicki, M. A. (2008). The emerging biology of satellite cells and their therapeutic potential. Trends Mol Med 14(2): 82-91. (Cover picture)
  27.  Kuang, S., Gillespie, M. A. and Rudnicki, M. A. (2008). Niche regulation of muscle satellite cell self-renewal and differentiation. Cell Stem Cell 2(1): 22-31.
  28. Seale, P., Bjork, B., Yang, W., Kajimura, S., Chin, S., Kuang, S., Scime, A., Devarakonda, S., Conroe, H. M., Erdjument-Bromage, H., Tempst, P., Rudnicki, M. A., Beier, D. R. and Spiegelman, B. M. (2008). PRDM16 controls a brown fat/skeletal muscle switch. Nature 454(7207): 961-967. (Article, Cover picture)
  29. Kuang, S., Kuroda, K., Le Grand, F. and Rudnicki, M. A. (2007). Asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell 129(5): 999-1010.
  30. Kuang, S., Charge, S. B., Seale, P., Huh, M. and Rudnicki, M. A. (2006). Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis. J Cell Biol 172(1): 103-113.

3 Protocols published
Muscle Histology Characterization Using H&E Staining and Muscle Fiber Type Classification Using Immunofluorescence Staining
Authors:  Chao Wang, Feng Yue and Shihuan Kuang, date: 05/20/2017, view: 4937, Q&A: 0
Muscle function is determined by its structure and fiber type composition. Here we describe a protocol to examine muscle histology and myofiber types using hematoxylin and eosin (H&E) and immunofluorescence staining, respectively. H&E stain nucleus ...
Evaluation of Muscle Performance in Mice by Treadmill Exhaustion Test and Whole-limb Grip Strength Assay
Authors:  Beatriz Castro and Shihuan Kuang, date: 04/20/2017, view: 3400, Q&A: 0
In vivo muscle function testing has become of great interest as primary phenotypic analysis of muscle performance. This protocol provides detailed procedures to perform the treadmill exhaustion test and the whole-limb grip strength assay, ...