Jiamin Qiu 1 protocol

Kun Kim 1 protocol

Jennifer Freeman 1 protocol

Feng Yue Department of Animal Science, Purdue University, USA
2 protocols

Shihuan Kuang
  • Faculty, Department of Animal Sciences, Department of Health & Kinesiology, Center for Cancer Research, Purdue University
Research focus
  • Developmental biology
  • Skeletal muscle and adipose tissue biology
  • 5 Author merit


Ph.D in Physiology and Cell Biology, Department of Biological Sciences, University of Alberta, Edmonton, Canada, 2002


• Shan, T., Zhang, P., Bi, P. and Kuang, S. (2015). Lkb1 deletion promotes ectopic lipid accumulation in muscle progenitor cells and mature muscles. J Cell Physiol 230(5): 1033-1041.
• Wang, J. H., Wang, Q. J., Wang, C., Reinholt, B., Grant, A. L., Gerrard, D. E. and Kuang, S. (2015). Heterogeneous activation of a slow myosin gene in proliferating myoblasts and differentiated single myofibers. Dev Biol 402(1): 72-80.
• Bi, P. and Kuang, S. (2015). Notch signaling as a novel regulator of metabolism. Trends Endocrinol Metab 26(5): 248-255.
• Yu, H., Waddell, J. N., Kuang, S. and Bidwell, C. A. (2014). Park7 expression influences myotube size and myosin expression in muscle. PLoS One 9(3): e92030.
• Jiang, C., Wen, Y., Kuroda, K., Hannon, K., Rudnicki, M. A. and Kuang, S. (2014). Notch signaling deficiency underlies age-dependent depletion of satellite cells in muscular dystrophy. Dis Model Mech 7(8): 997-1004.
• Shan, T., Zhang, P., Liang, X., Bi, P., Yue, F. and Kuang, S. (2014). Lkb1 is indispensable for skeletal muscle development, regeneration, and satellite cell homeostasis. Stem Cells 32(11): 2893-2907.
• Zhang, P., Shan, T., Liang, X., Deng, C. and Kuang, S. (2014). Mammalian target of rapamycin is essential for cardiomyocyte survival and heart development in mice. Biochem Biophys Res Commun 452(1): 53-59.
• Bi, P., Shan, T., Yang, X., Liu, W., Yue, F., Liang, X., Wang, J., Li, J,, Carlesso. N,, Liu. X. and Kuang, S. (2014). Notch signaling regulates adipose browning and energy metabolism. Nat Med 20(8): 911-8.
• Shan, T., Liu, W. and Kuang, S. (2013). Fatty acid binding protein 4 expression marks a population of adipocyte progenitors in white and brown adipose tissues. FASEB J 27(1): 277-287.
• Shan, T., Liang, X., Bi, P. and Kuang, S. (2013). Myostatin knockout drives browning of white adipose tissue through activating the AMPK-PGC1alpha-Fndc5 pathway in muscle. FASEB J27(5): 1981-1989.
• Kuroda, K., Kuang, S., Taketo, M. M. and Rudnicki, M. A. (2013). Canonical Wnt signaling induces BMP-4 to specify slow myofibrogenesis of fetal myoblasts. Skelet Muscle 3(1): 5.
• Liu, W., Bi, P., Shan, T., Yang, X., Yin, H., Wang, Y. X., Liu, N., Rudnicki, M. A. and Kuang, S. (2013). miR-133a regulates adipocyte browning in vivo. PLoS Genet 9(7): e1003626.
• Shan, T., Liang, X., Bi, P., Zhang, P., Liu, W. and Kuang, S. (2013). Distinct populations of adipogenic and myogenic Myf5-lineage progenitors in white adipose tissues. J Lipid Res 54(8): 2214-2224.
• Liu, W., Shan, T., Yang, X., Liang, S., Zhang, P., Liu, Y., Liu, X. and Kuang, S. (2013). A heterogeneous lineage origin underlies the phenotypic and molecular differences of white and beige adipocytes. J Cell Sci 126(Pt 16): 3527-3532.
• Ogura, Y., Mishra, V., Hindi, S. M., Kuang, S. and Kumar, A. (2013). Proinflammatory cytokine tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) suppresses satellite cell self-renewal through inversely modulating Notch and NF-kappaB signaling pathways. J Biol Chem 288(49): 35159-35169.
• Chakkalakal, J. V., Kuang, S., Buffelli, M., Lichtman, J. W. and Sanes, J. R. (2012). Mouse transgenic lines that selectively label Type I, Type IIA, and Types IIX+B skeletal muscle fibers.Genesis 50(1): 50-58.
• Wen, Y., Bi, P., Liu, W., Asakura, A., Keller, C. and Kuang, S. (2012). Constitutive Notch activation upregulates Pax7 and promotes the self-renewal of skeletal muscle satellite cells. Mol Cell Biol32(12): 2300-2311.
• Liu, W., Liu, Y., Lai, X. and Kuang, S. (2012). Intramuscular adipose is derived from a non-Pax3 lineage and required for efficient regeneration of skeletal muscles. Dev Biol 361(1): 27-38.
• Liu, W., Wen, Y., Bi, P., Lai, X., Liu, X. S., Liu, X. and Kuang, S. (2012). Hypoxia promotes satellite cell self-renewal and enhances the efficiency of myoblast transplantation. Development139(16): 2857-2865.
• Hindi, S. M., Paul, P. K., Dahiya, S., Mishra, V., Bhatnagar, S., Kuang, S., Choi, Y. and Kumar, A. (2012). Reciprocal interaction between TRAF6 and notch signaling regulates adult myofiber regeneration upon injury. Mol Cell Biol 32(23): 4833-4845.
• Dahiya, S., Bhatnagar, S., Hindi, S. M., Jiang, C., Paul, P. K., Kuang, S. and Kumar, A. (2011). Elevated levels of active matrix metalloproteinase-9 cause hypertrophy in skeletal muscle of normal and dystrophin-deficient mdx mice. Hum Mol Genet 20(22): 4345-4359.
• Angione, A. R., Jiang, C., Pan, D., Wang, Y. X. and Kuang, S. (2011). PPARdelta regulates satellite cell proliferation and skeletal muscle regeneration. Skelet Muscle 1(1): 33.
• Mittal, A., Bhatnagar, S., Kumar, A., Paul, P. K., Kuang, S. and Kumar, A. (2010). Genetic ablation of TWEAK augments regeneration and post-injury growth of skeletal muscle in mice. Am J Pathol 177(4): 1732-1742.
• Waddell, J. N., Zhang, P., Wen, Y., Gupta, S. K., Yevtodiyenko, A., Schmidt, J. V., Bidwell, C. A., Kumar, A. and Kuang, S. (2010). Dlk1 is necessary for proper skeletal muscle development and regeneration. PLoS One 5(11): e15055.
• Gillespie, M. A., Le Grand, F., Scime, A., Kuang, S., von Maltzahn, J., Seale, V., Cuenda, A., Ranish, J. A. and Rudnicki, M. A. (2009). p38-{gamma}-dependent gene silencing restricts entry into the myogenic differentiation program. J Cell Biol 187(7): 991-1005.
• Kuang, S. and Rudnicki, M. A. (2008). The emerging biology of satellite cells and their therapeutic potential. Trends Mol Med 14(2): 82-91. (Cover picture)
• Kuang, S., Gillespie, M. A. and Rudnicki, M. A. (2008). Niche regulation of muscle satellite cell self-renewal and differentiation. Cell Stem Cell 2(1): 22-31.
• Seale, P., Bjork, B., Yang, W., Kajimura, S., Chin, S., Kuang, S., Scime, A., Devarakonda, S., Conroe, H. M., Erdjument-Bromage, H., Tempst, P., Rudnicki, M. A., Beier, D. R. and Spiegelman, B. M. (2008). PRDM16 controls a brown fat/skeletal muscle switch. Nature 454(7207): 961-967. (Article, Cover picture)
• Kuang, S., Kuroda, K., Le Grand, F. and Rudnicki, M. A. (2007). Asymmetric self-renewal and commitment of satellite stem cells in muscle. Cell 129(5): 999-1010.
• Kuang, S., Charge, S. B., Seale, P., Huh, M. and Rudnicki, M. A. (2006). Distinct roles for Pax7 and Pax3 in adult regenerative myogenesis. J Cell Biol 172(1): 103-113.
6 Protocols published
Authors:  Kun Ho Kim, Jiamin Qiu and Shihuan Kuang, date: 07/20/2020, view: 3762, Q&A: 0
The skeletal muscle is key for body mobility and motor performance, but aging and diseases often lead to progressive loss of muscle mass due to wasting or degeneration of muscle cells. Muscle satellite cells (MuSCs) represent a population of tissue ...
This protocol details a method to analyze two tissue samples at the transcriptomic level using microarray analysis, ingenuity pathway analysis (IPA) and gene set enrichment analysis (GSEA). Methods such as these provide insight into the mechanisms ...
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