One possibility is that your conidiospore solution contains contamination of other pathogens (e.g. necrotrophs).
Avoid any soil contaminations when you harvest aerial parts of the infected plants because it may cause propagation of soil inhabiting pathogens.
Are you sure the plant is a Col-0 pad4 mutant?, you use water without any surfactants?
Hope that your experiment goes well.
4/23/2018 8:59:54 PM Reply