Robin Farhaeus Cibles Therapeutiques, INSERM Unite 940, Universite Paris 7, France
2 protocols
Antoine de Morree Stanford university
33 protocols

HongLok Lung University of Hong Kong
59 protocols

Isabel Silva University of São Paulo State - UNESP
6 protocols

Lee-Hwa Tai Universite de Sherbrooke
20 protocols

Reviewer
Vanesa Olivares-Illana
  • Research scientist, Instituto de Física, Universidad Autónoma de San Luis Potosí
Research focus
  • Cancer biology
  • Biochemistry, cancer, interactions, retinoblastoma, p53
  • 2 Author merit

Education

PhD, UNAM, 2003

Lab information

Vanesa Olivares Illana Labs

2 Protocols published
Purification of His-ubiquitin Proteins from Mammalian Cells
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 14451, Q&A: 7
This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just ...
Protein-RNA ELISA Assay
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 8654, Q&A: 0
Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.
13 Protocols reviewed
Induction of Connexin-hemichannel Opening
Authors:  Andrea Puhar and Philippe J. Sansonetti, date: 09/05/2014, view: 4967, Q&A: 0
Connexins (Cxs) are integral membrane proteins of vertebrates that associate to form hexameric transmembrane channels, named hemichannels. Twenty-one Cx types have been described, which are named according to their molecular weight. Cxs are ...
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3D Mammary Colony-Forming Cell Assay
Authors:  Giusy Tornillo and Sara Cabodi, date: 04/05/2014, view: 10297, Q&A: 0
The mammary epithelium consists of multiple phenotypically and functionally distinct cell populations, which are organized as a hierarchy of stem cells, progenitors and terminally differentiated cells.

Identification of the mechanisms ...
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