Aksiniya Asenova University of California, Davis
12 protocols

Alexandros Alexandratos National and Kapodistrian University of Athens
11 protocols

Alka Mehra Institute of Genomics and Integrative Biology
21 protocols

Amit Dey Regional Centre for Biotechnology
10 protocols

Editor
Fanglian He
  • Editor, Bio-protocol
Research focus
  • Microbiology
  • Host-bacterium interaction
  • 19 Author merit

Education

Ph.D., University of Pennsylvania, USA

19 Protocols published
Alkaline Phosphatase
Author:  Fanglian He, date: 07/20/2012, view: 21450, Q&A: 2
Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.
Oil Red O Staining of Fixed Worm
Author:  Fanglian He, date: 07/05/2012, view: 18634, Q&A: 1
Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.
63 Protocols reviewed
Rice Meiotic Chromosome Spread Preparation of Pollen Mother Cells
Authors:  Xingwang Li and Changyin Wu, date: 07/20/2014, view: 6057, Q&A: 0
In this protocol, we describe a simple and efficient method for meiotic chromosome spread preparation in rice pollen mother cells. Meiotic chromosome preparation by spreading itself is an important technique for plant cytogenetics (Higgins et al. ...
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Novel Method for Site-specific Induction of Oxidative DNA Damage to Study Recruitment of Repair Proteins to Heterochromatin and Euchromatin
Authors:  Leizhen Wei, Satoshi Nakajima, Arthur S. Levine and Li Lan, date: 06/05/2014, view: 5809, Q&A: 0
ROS-induced DNA damage is repaired in living cells within a temporal and spatial context, and chromatin structure is critical to a consideration of DNA repair processes in situ. It’s well known that chromatin remodeling factors participate ...
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142 Protocols edited
Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting
Authors:  Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 10714, Q&A: 0
In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...
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Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae
Authors:  David Canadell, Samuel Bru, Josep Clotet and Joaquín Ariño, date: 07/20/2016, view: 5852, Q&A: 0
Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond ...
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