Hello,
Could you explain the purpose of the MRWB buffer? Some papers use it or PBS indistinguishly. Why use spermine and spermidine?
Why is the reduction buffer necessary? It is not used by O'Rourke (O'Rourke et al., 2009)
Thank you.
Regards
David Buj
PhD student
University of Huddersfield
O'Rourke, E. J., Soukas, A. A., Carr, C. E. and Ruvkun, G. (2009). C. elegans major fats are stored in vesicles distinct from lysosome-related organelles. Cell Metab 10(5): 430-435
12/7/2015 6:42:47 AM Reply