Fanglian He —BIO-PROTOCOL

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Coreviewers

Aksiniya Asenova University of California, Davis
11 protocols

Alexandros Alexandratos University of Athens
10 protocols

Alka Mehra Institute of Genomics and Integrative Biology
18 protocols

Amit Dey Regional Centre for Biotechnology
10 protocols

Editor

Fanglian He

Editor, University of Pennsylvania

Research focus

Microbiology
Host-bacterium interaction, Agrobacterium, C. elegans

19 Author merit
Reviewer merit
Editor merit

Education

Ph.D., University of Pennsylvania, USA

19 Protocols published

Alkaline Phosphatase

Author: Fanglian He, date: 07/20/2012, view: 16996, Q&A: 2

Alkaline phosphatase removes 5' phosphate groups from vector so that prevents self-ligation of the vector and facilitates the ligation of other DNA fragments into the vector.

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Oil Red O Staining of Fixed Worm

Author: Fanglian He, date: 07/05/2012, view: 16300, Q&A: 1

Oil red O staining is used to assess major fat stores in C. elegans. This protocol is adapted from the Ashrafi Lab at the University of California-San Francisco.

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63 Protocols reviewed

Quantitation of Cytochromes b₅₅₉, b₆, and f, and the Core Component of Photosystem I P₇₀₀ in Cyanobacterial Cells

Authors: Motohide Aoki, Mikio Tsuzuki and Norihiro Sato, date: 11/05/2016, view: 2625, Q&A: 0

Cytochrome (Cyt) b₅₅₉, an important and essential core component of photosystem II in the photosynthetic electron transport system, is a heme-bridged heterodimer protein composed of an alpha subunit (PsbE) and a beta ...

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Quantification of Callose Deposition in Plant Leaves

Authors: Loredana Scalschi, Eugenio Llorens, Gemma Camañes, Victoria Pastor, Emma Fernández-Crespo, Victor Flors, Pilar García-Agustín and Begonya Vicedo, date: 10/05/2015, view: 5103, Q&A: 0

Callose is an amorphous homopolymer, composed of β-1, 3-glucan, which is widespread in higher plants. Callose is involved in multiple aspects of plant growth and development. It is synthetized in plants at the cell plate during cytokinesis, in ...

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142 Protocols edited

Isolation of Highly Pure Primary Mouse Alveolar Epithelial Type II Cells by Flow Cytometric Cell Sorting

Authors: Meenal Sinha and Clifford A. Lowell, date: 11/20/2016, view: 7819, Q&A: 0

In this protocol, we describe the method for isolating highly pure primary alveolar epithelial type II (ATII) cells from lungs of naïve mice. The method combines negative selection for a variety of lineage markers along with positive selection for ...

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Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae

Authors: David Canadell, Samuel Bru, Josep Clotet and Joaquín Ariño, date: 07/20/2016, view: 4298, Q&A: 0

Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond ...

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Anaida Walia

Amity Institute of Biotechnology

Fanglian He

University of Pennsylvania

David Buj

University of Huddersfield