Éva Kiss 1 protocol

Petra Skotnicová 1 protocol

Minna Koskela 1 protocol

Judith Noda Institute of Microbiology, Academy of Sciences, Czech Republic
1 protocol

Roman Sobotka
  • Senior Scientist, Laboratory of Photosynthesis, Institute of Microbiology, Třeboň
Research focus
  • Plant science
  • 3 Author merit


Ph.D. in Plant Genetics, Faculty of Agriculture, University of South Bohemia, České Budějovice, Czech Republic, 2001


Since 2005
• Knoppova, J., Sobotka, R., Tichy, M., Yu, J., Konik, P., Halada, P., Nixon, P. J. and Komenda, J. (2014). Discovery of a chlorophyll binding protein complex involved in the early steps of photosystem II assembly in Synechocystis. Plant Cell 26(3): 1200-1212.
• Chidgey, J. W., Linhartova, M., Komenda, J., Jackson, P. J., Dickman, M. J., Canniffe, D. P., Konik, P., Pilny, J., Hunter, C. N. and Sobotka, R. (2014). A cyanobacterial chlorophyll synthase-HliD complex associates with the Ycf39 protein and the YidC/Alb3 insertase. Plant Cell 26(3): 1267-1279.
• Sobotka, R. (2014). Making proteins green; biosynthesis of chlorophyll-binding proteins in cyanobacteria. Photosynth Res 119(1-2): 223-232.
• Kopečná, J., Sobotka, R. and Komenda, J. (2013). Inhibition of chlorophyll biosynthesis at the protochlorophyllide reduction step results in the parallel depletion of Photosystem I and Photosystem II in the cyanobacterium Synechocystis PCC 6803. Planta 237(2): 497-508.
• Kopecna, J., Komenda, J., Bucinska, L. and Sobotka, R. (2012). Long-term acclimation of the cyanobacterium Synechocystis sp. PCC 6803 to high light is accompanied by an enhanced production of chlorophyll that is preferentially channeled to trimeric photosystem I. Plant Physiol 160(4): 2239-2250.
• Hollingshead, S., Kopecna, J., Jackson, P. J., Canniffe, D. P., Davison, P. A., Dickman, M. J., Sobotka, R. and Hunter, C. N. (2012). Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803. J Biol Chem 287(33): 27823-27833.
• Wallner, T., Hagiwara, Y., Bernat, G., Sobotka, R., Reijerse, E. J., Frankenberg-Dinkel, N. and Wilde, A. (2012). Inactivation of the conserved open reading frame ycf34 of Synechocystis sp. PCC 6803 interferes with the photosynthetic electron transport chain. Biochim Biophys Acta 1817(11): 2016-2026.
• Komenda, J., Sobotka, R. and Nixon, P. J. (2012). Assembling and maintaining the Photosystem II complex in chloroplasts and cyanobacteria. Curr Opin Plant Biol 15(3): 245-251.
• Koreny, L., Sobotka, R., Kovarova, J., Gnipova, A., Flegontov, P., Horvath, A., Obornik, M., Ayala, F. J. and Lukes, J. (2012). Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability. Proc Natl Acad Sci U S A 109(10): 3808-3813.
• Kana, R., Kotabova, E., Sobotka, R. and Prasil, O. (2012). Non-photochemical quenching in cryptophyte alga Rhodomonas salina is located in chlorophyll a/c antennae. PLoS One 7(1): e29700.
• Komenda, J., Knoppova, J., Kopecna, J., Sobotka, R., Halada, P., Yu, J., Nickelsen, J., Boehm, M. and Nixon, P. J. (2012). The Psb27 assembly factor binds to the CP43 complex of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol 158(1): 476-486.
• Koreny, L., Sobotka, R., Janouskovec, J., Keeling, P. J. and Obornik, M. (2011). Tetrapyrrole synthesis of photosynthetic chromerids is likely homologous to the unusual pathway of apicomplexan parasites. Plant Cell 23(9): 3454-3462.
• Sobotka, R., Tichy, M., Wilde, A. and Hunter, C. N. (2011). Functional assignments for the carboxyl-terminal domains of the ferrochelatase from Synechocystis PCC 6803: the CAB domain plays a regulatory role, and region II is essential for catalysis. Plant Physiol 155(4): 1735-1747.
• Dobakova, M., Sobotka, R., Tichy, M. and Komenda, J. (2009). Psb28 protein is involved in the biogenesis of the photosystem II inner antenna CP47 (PsbB) in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol 149(2): 1076-1086.
• Sobotka, R., Duhring, U., Komenda, J., Peter, E., Gardian, Z., Tichy, M., Grimm, B. and Wilde, A. (2008). Importance of the cyanobacterial Gun4 protein for chlorophyll metabolism and assembly of photosynthetic complexes. J Biol Chem283(38): 25794-25802.
• Sobotka, R., McLean, S., Zuberova, M., Hunter, C. N. and Tichy, M. (2008). The C-terminal extension of ferrochelatase is critical for enzyme activity and for functioning of the tetrapyrrole pathway in Synechocystis strain PCC 6803. J Bacteriol190(6): 2086-2095.
• Sobotka, R., Komenda, J., Bumba, L. and Tichy, M. (2005). Photosystem II assembly in CP47 mutant of Synechocystis sp. PCC 6803 is dependent on the level of chlorophyll precursors regulated by ferrochelatase. J Biol Chem 280(36): 31595-31602.
3 Protocols published
Purification of Protein-complexes from the Cyanobacterium Synechocystis sp. PCC 6803 Using FLAG-affinity Chromatography
Authors:  Minna M. Koskela, Petra Skotnicová, Éva Kiss and Roman Sobotka, date: 05/20/2020, view: 2492, Q&A: 0
Exploring the structure and function of protein complexes requires their isolation in the native state–a task that is made challenging when studying labile and/or low abundant complexes. The difficulties in preparing membrane-protein complexes are ...
Detection and Quantification of Heme and Chlorophyll Precursors Using a High Performance Liquid Chromatography (HPLC) System Equipped with Two Fluorescence Detectors
Authors:  Jan Pilný, Jana Kopečná, Judith Noda and Roman Sobotka, date: 02/05/2015, view: 10346, Q&A: 0
Intermediates of tetrapyrrole biosynthetic pathway are low-abundant compounds, and their quantification is usually difficult, time consuming and requires large amounts of input material. Here, we describe a method allowing fast and accurate ...
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