hello Dear thank you very much for the protocol . please i have questions . i want estimate H2O2 in wheat leaves stressed with fungi. so in what time i must collect the leaves? means wich day that the hydrogen peroxyde will be in his hight level after plant stressing. the second question is that i have this protocol : Hydrogen peroxide levels were determined ac-cording to Sergiev et al. [10]. Leaf tissues (500 mg)were homogenized in ice bath with 5 ml 0.1%(w:v) TCA. The homogenate was centrifuged at12 000gfor 15 min and 0.5 ml of the superna-tant was added to 0.5 ml 10 mM potassium phos-phate buffer (pH 7.0) and 1 ml 1 M KI. Theabsorbancy of supernatant was read at 390 nm.The content of H2O2was given on a standardcurve. this protocol is very simple but i descute with professor, he said me that he had problem with this one. so what is your opinion? 2/23/2019 10:27:08 AM Reply