Dawn Bignell Department of Biology, Memorial University of Newfoundland, Canada
1 protocol

Luke Bown
  • Ph.D Student, Memorial University of Newfoundland
Research focus
  • Microbiology
  • Streptomyces, natural products
  • 1 Author merit


B. Sc. (Hons), Memorial University of Newfoundland, 2014


Bown, L. †, Srivastava, S.K. †, Piercey, B.M. †, McIsaac, C.K., Tahlan, K. (2017). Mycobacterial Membrane Proteins QcrB and AtpE: Roles in Energetics, Antibiotic Targets and Associated Mechanisms of Resistance. The Journal of Membrane Biology: 1-13. (†Co-first authors)

Bown, L. & Bignell, D.R.D. (2017). Draft Genome Sequence of the Plant Pathogen Streptomyces sp. 11-1-2. Genome Announcements 5(37): e00968-17.

Bown,L., Li, Y., Berrue, F., Verhoeven, J., Dufour, S. and Bignell, D. (2017). Biosynthesis and evolution of coronafacoyl phytotoxin production in the common scab pathogen Streptomyces scabies. Applied and Environmental Microbiology, AEM-01169.

Bown, L. & Bignell, D.R. (2017). Purification of N-coronafacoyl Phytotoxins from Streptomyces scabies. Bio-protocol 7(7): e2214. DOI: 10.21769/BioProtoc.2214.

Hynes, A., Shakya, M., Mercer, R., Grüll, M., Bown, L., Davidson, F., Steffen, E., Matchem, H., Peach, M., Berger, T., Grebe, K., Zhaxybayeva, O. & Lang, A. (2016). Functional and evolutionary characterization of a gene transfer agent’s multi-locus “genome”. Molecular Biology and Evolution, 33(10): 2530-2543.

Bown, L.†, Altowairish, M.†, Fyans, J.K. & Bignell, D.R.D. (2016). Production of the Streptomyces scabies coronafacoyl phytotoxins involves a novel biosynthetic pathway with an F420-dependent oxidoreductase and a short-chain dehydrogenase/reductase. Molecular Microbiology, 101(1): 122-135. (†Co-first authors)

Fyans, J.K.†, Bown, L.† & Bignell, D.R.D. (2016). Isolation and characterization of plant pathogenic Streptomyces species associated with common scab-infected potato tubers in Newfoundland. Phytopathology, 106(2): 123-131. (†Co-first authors)

Cheng, Z., Bown, L., Tahlan, K. & Bignell, D.R.D. (2015). Regulation of coronafacoyl phytotoxin production by the PAS-LuxR family regulator CfaR in the common scab pathogen Streptomyces scabies. Plos ONE, 10(3): e0122450.
1 Protocol published
Purification of N-coronafacoyl Phytotoxins from Streptomyces scabies
Authors:  Luke Bown and Dawn R. D. Bignell, date: 04/05/2017, view: 2701, Q&A: 0
This procedure is used for large-scale purification of N-coronafacoyl phytotoxins that are produced by the potato common scab pathogen Streptomyces scabies. The procedure employs organic extraction of S. scabies culture ...