Andrea Dorfleutner Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, USA
2 protocols

Lucia de Almeida Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, USA
2 protocols

Christian Stehlik
  • Division of Rheumatology, Department of Medicine, Feinberg School of Medicine, Northwestern University, USA
  • 2 Author merit

Education

Ph.D. in Immunology and Biochemistry, University of Vienna, Austria, 1999

Current position

Associate Professor of Medicine, Department of Medicine/Division of Rheumatology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA

Publications (since 2007)

  1. Dorfleutner, A. and Stehlik, C. (2016). A dRAStic RHOAdblock of Pyrin inflammasome activation. Nat Immunol 17(8): 900-902.

  2. Ratsimandresy, R., Indramohan, M., Dorfleutner, A. and Stehlik, C. (2016) The AIM2 inflammasome is a central regulator of intestinal homeostasis through the IL-18/IL-22/STAT3 pathway. Cellular & Molecular Immunology.
  3. Khare, S., Radian, A. D., Dorfleutner, A. and Stehlik, C. (2016). Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking. Methods Mol Biol 1417: 131-143.
  4. Cuda, C. M., Misharin, A. V., Khare, S., Saber, R., Tsai, F., Archer, A. M., Homan, P. J., Haines, G. K., 3rd, Hutcheson, J., Dorfleutner, A., Budinger, G. R., Stehlik, C. and Perlman, H. (2015). Conditional deletion of caspase-8 in macrophages alters macrophage activation in a RIPK-dependent manner. Arthritis Res Ther 17: 291.
  5. Radian, A. D., Khare, S., Chu, L. H., Dorfleutner, A. and Stehlik, C. (2015). ATP binding by NLRP7 is required for inflammasome activation in response to bacterial lipopeptides. Mol Immunol 67(2 Pt B): 294-302.
  6. de Almeida, L., Khare, S., Misharin, A. V., Patel, R., Ratsimandresy, R. A., Wallin, M. C., Perlman, H., Greaves, D. R., Hoffman, H. M., Dorfleutner, A. and Stehlik, C. (2015). The PYRIN Domain-only Protein POP1 Inhibits Inflammasome Assembly and Ameliorates Inflammatory Disease. Immunity 43(2): 264-276.
  7. Dorfleutner, A., Chu, L. and Stehlik, C. (2015). Inhibiting the inflammasome: one domain at a time. Immunol Rev 265(1): 205-216.
  8. Chu, L. H., Gangopadhyay, A., Dorfleutner, A. and Stehlik, C. (2015). An updated view on the structure and function of PYRIN domains. Apoptosis 20(2): 157-173.
  9. Thorp, E. B., Stehlik, C. and Ansari, M. J. (2015). T-cell exhaustion in allograft rejection and tolerance. Curr Opin Organ Transplant 20(1): 37-42.
  10. Queen, J., Agarwal, S., Dolores, J. S., Stehlik, C. and Satchell, K. J. (2015). Mechanisms of inflammasome activation by Vibrio cholerae secreted toxins vary with strain biotype. Infect Immun 83(6): 2496-2506.
  11. Talbott, S. J., Luanpitpong, S., Stehlik, C., Azad, N., Iyer, A. K., Wang, L. and Rojanasakul, Y. (2014). S-nitrosylation of FLICE inhibitory protein determines its interaction with RIP1 and activation of NF-kappaB. Cell Cycle 13(12): 1948-1957.
  12. Cuda, C. M., Misharin, A. V., Gierut, A. K., Saber, R., Haines, G. K., 3rd, Hutcheson, J., Hedrick, S. M., Mohan, C., Budinger, G. S., Stehlik, C. and Perlman, H. (2014). Caspase-8 acts as a molecular rheostat to limit RIPK1- and MyD88-mediated dendritic cell activation. J Immunol 192(12): 5548-5560.
  13. Khare, S., Ratsimandresy, R. A., de Almeida, L., Cuda, C. M., Rellick, S. L., Misharin, A. V., Wallin, M. C., Gangopadhyay, A., Forte, E., Gottwein, E., Perlman, H., Reed, J. C., Greaves, D. R., Dorfleutner, A. and Stehlik, C. (2014). The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses. Nat Immunol 15(4): 343-353.
  14. Ratsimandresy, R. A., Dorfleutner, A. and Stehlik, C. (2013). An Update on PYRIN Domain-Containing Pattern Recognition Receptors: From Immunity to Pathology. Front Immunol 4: 440.
  15. Radian, A. D., de Almeida, L., Dorfleutner, A. and Stehlik, C. (2013). NLRP7 and related inflammasome activating pattern recognition receptors and their function in host defense and disease. Microbes Infect 15(8-9): 630-639.
  16. Luanpitpong, S., Chanvorachote, P., Stehlik, C., Tse, W., Callery, P. S., Wang, L. and Rojanasakul, Y. (2013). Regulation of apoptosis by Bcl-2 cysteine oxidation in human lung epithelial cells. Mol Biol Cell 24(6): 858-869.
  17. Shi, B., Huang, Q., Tak, P. P., Vervoordeldonk, M. J., Huang, C. C., Dorfleutner, A., Stehlik, C. and Pope, R. M. (2012). SNAPIN: an endogenous Toll-like receptor ligand in rheumatoid arthritis. Ann Rheum Dis 71(8): 1411-1417.
  18. Luanpitpong, S., Chanvorachote, P., Nimmannit, U., Leonard, S. S., Stehlik, C., Wang, L. and Rojanasakul, Y. (2012). Mitochondrial superoxide mediates doxorubicin-induced keratinocyte apoptosis through oxidative modification of ERK and Bcl-2 ubiquitination. Biochem Pharmacol 83(12): 1643-1654.
  19. Huang, Q. Q., Koessler, R. E., Birkett, R., Dorfleutner, A., Perlman, H., Haines, G. K., 3rd, Stehlik, C., Nicchitta, C. V. and Pope, R. M. (2012). Glycoprotein 96 perpetuates the persistent inflammation of rheumatoid arthritis. Arthritis Rheum 64(11): 3638-3648.
  20. Khare, S., Dorfleutner, A., Bryan, N. B., Yun, C., Radian, A. D., de Almeida, L., Rojanasakul, Y. and Stehlik, C. (2012). An NLRP7-containing inflammasome mediates recognition of microbial lipopeptides in human macrophages. Immunity 36(3): 464-476.
  21. Kung, V. L., Khare, S., Stehlik, C., Bacon, E. M., Hughes, A. J. and Hauser, A. R. (2012). An rhs gene of Pseudomonas aeruginosa encodes a virulence protein that activates the inflammasome. Proc Natl Acad Sci U S A 109(4): 1275-1280.
  22. Iyer, A. K., Azad, N., Talbot, S., Stehlik, C., Lu, B., Wang, L. and Rojanasakul, Y. (2011). Antioxidant c-FLIP inhibits Fas ligand-induced NF-kappaB activation in a phosphatidylinositol 3-kinase/Akt-dependent manner. J Immunol 187(6): 3256-3266.
  23. Khare, S., Luc, N., Dorfleutner, A. and Stehlik, C. (2010). Inflammasomes and their activation. Crit Rev Immunol 30(5): 463-487.
  24. Azad, N., Iyer, A., Vallyathan, V., Wang, L., Castranova, V., Stehlik, C. and Rojanasakul, Y. (2010). Role of oxidative/nitrosative stress-mediated Bcl-2 regulation in apoptosis and malignant transformation. Ann N Y Acad Sci 1203: 1-6.
  25. Bryan, N. B., Dorfleutner, A., Kramer, S. J., Yun, C., Rojanasakul, Y. and Stehlik, C. (2010). Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes. J Inflamm (Lond) 7: 23.
  26. Stehlik, C. (2009). Multiple interleukin-1beta-converting enzymes contribute to inflammatory arthritis. Arthritis Rheum 60(12): 3524-3530.
  27. Bryan, N. B., Dorfleutner, A., Rojanasakul, Y. and Stehlik, C. (2009). Activation of inflammasomes requires intracellular redistribution of the apoptotic speck-like protein containing a caspase recruitment domain. J Immunol 182(5): 3173-3182.
  28. Dorfleutner, A., Cho, Y., Vincent, D., Cunnick, J., Lin, H., Weed, S. A., Stehlik, C. and Flynn, D. C. (2008). Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells. J Cell Sci 121(Pt 14): 2394-2405.
  29. Wang, L., Chanvorachote, P., Toledo, D., Stehlik, C., Mercer, R. R., Castranova, V. and Rojanasakul, Y. (2008). Peroxide is a key mediator of Bcl-2 down-regulation and apoptosis induction by cisplatin in human lung cancer cells. Mol Pharmacol 73(1): 119-127.
  30. Stehlik, C. and Dorfleutner, A. (2007). COPs and POPs: modulators of inflammasome activity. J Immunol 179(12): 7993-7998.
  31. Stehlik, C. (2007). The PYRIN domain in signal transduction. Curr Protein Pept Sci 8(3): 293-310.
  32. Dorfleutner, A., Stehlik, C., Zhang, J., Gallick, G. E. and Flynn, D. C. (2007). AFAP-110 is required for actin stress fiber formation and cell adhesion in MDA-MB-231 breast cancer cells. J Cell Physiol 213(3): 740-749.
  33. Dorfleutner, A., Bryan, N. B., Talbott, S. J., Funya, K. N., Rellick, S. L., Reed, J. C., Shi, X., Rojanasakul, Y., Flynn, D. C. and Stehlik, C. (2007). Cellular pyrin domain-only protein 2 is a candidate regulator of inflammasome activation. Infect Immun 75(3): 1484-1492.
  34. Dorfleutner, A., Talbott, S. J., Bryan, N. B., Funya, K. N., Rellick, S. L., Reed, J. C., Shi, X., Rojanasakul, Y., Flynn, D. C. and Stehlik, C. (2007). A Shope Fibroma virus PYRIN-only protein modulates the host immune response. Virus Genes 35(3): 685-694.
2 Protocols published
In vivo Analysis of Neutrophil Infiltration during LPS-induced Peritonitis
Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 4409, Q&A: 0
Bacterial lipopolysaccharide (LPS) is present in the outer membrane of Gram-negative bacteria and functions as pathogen-associated molecular pattern (PAMP) (Whitfield and Trent, 2014). LPS therefore is a potent activator of inflammatory responses ...
ASC-particle-induced Peritonitis
Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 2900, Q&A: 0
In response to pathogen infection and tissue damage, inflammasome sensors such as NLRP3 and AIM2 are activated, which triggers PYRIN domain (PYD)-mediated ASC nucleation, followed by self-perpetuating ASC polymerization, which ultimately culminates ...
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