Massimiliano Gnecchi Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo, Italy
1 protocol
Antoine de Morree Stanford university
31 protocols

Ningfei An University of Chicago
6 protocols

Vivien Coulson-Thomas University of Houston
17 protocols

Federica Pisano
  • Post-Doc, Department of Cardiothoracic and Vascular Sciences-Coronary Care Unit and Laboratory of Clinical and Experimental Cardiology-Fondazione IRCCS Policlinico San Matteo
Research focus
  • Stem cell
  • mesenchymal stromal cells, acute miocardial infarction, regeneration
  • 1 Author merit


PhD in Bioengineering and Bioinformatics, University of Pavia, 2014

Lab information

Stem cell lab - molecular medicine department


Pisano Federica, Mura Manuela, Ciuffreda Maria Chiara, Calabrò Federica, Lanzo Nicola, Gnecchi Massimiliano. Optimized lentiviral transduction of human amniotic mesenchymal stromal cells. Pharmacological Research, [in press].

Manuela Mura, Ashish Mehta, Chrishan J. Ramachandra, Rita Zappatore, Federica Pisano, Maria Chiara Ciuffreda, Vincenzo Barbaccia, Lia Crotti, Peter J. Schwartz, Winston Shim, Massimiliano Gnecchi. The KCNH2-IVS9-28A/G mutation causes aberrant isoform expression and hERG trafficking defect in cardiomyocytes derived from patients affected by Long QT Syndrome type 2. Int J Cardiol. 2017 Aug 1;240:367-371.

Pisano F, Gnecchi M. Transfection of Embryoid Bodies with miRNA Precursors to Differentiate Cells into Cardiomyocytes.Bio-protocol, 2016.

Gnecchi M, Pisano F, Bariani R. MicroRNA and cardiac regeneration. MicroRNA: basic science. Adv Exp Med Biol. 2015;887:119-41. doi: 10.1007/978-3-319-22380-3_7.

Pisano F, Altomare C, Cervio E, Barile L, Rocchetti M, Ciuffreda M C, Malpasso G, Copes F, Mura M, Danieli P, Viarengo G, Zaza A, Gnecchi M. Combination of miRNA499 and miRNA133 exerts a synergic effect on cardiac differentiation. Stem Cells. 2015 Apr; 33(4):1187-99. doi: 10.1002/stem.1928.

Danieli P, Malpasso G, Ciuffreda MC, Cervio E, Calvillo L, Copes F, Pisano F, Mura M, Kleijn L, de Boer RA, Viarengo G, Rosti V, Spinillo A, Roccio M, Gnecchi M. Conditioned Medium From Human Amniotic Mesenchymal Stromal Cells Limits Infarct Size and Enhances Angiogenesis. Stem Cells Transl Med. 2015 Mar 30. pii: sctm.2014-0253. Stem Cells Translational Medicine 2015;4:1-11.

I. Del Vecchio, A. Zuccotti, F. Pisano, Canneva, S.C. Lenzken, F. Rousset, E. Corsini, S. Govoni, M. Racchi, Functional Mapping of the Promoter Region of the GNB2L1 Human Gene Coding for RACK1 Scaffold Protein. Gene 2009; 430: 17-29.

1 Protocol published
Transfection of Embryoid Bodies with miRNA Precursors to Induce Cardiac Differentiation
Authors:  Federica Pisano and Massimiliano Gnecchi, date: 02/05/2016, view: 3284, Q&A: 1
In recent years, the utilization of stem cell therapy to regenerate cardiac tissue has been proposed as a possible strategy to treat cardiac damage (Gnecchi et al., 2012, Aguirre et al., 2013; Sanganalmath and Bolli, 2013). ...
4 Protocols reviewed
Isolation and Primary Culture of Adult Human Adipose-derived Stromal/Stem Cells
Authors:  Robert B. Jones, Amy L. Strong, Jeffrey M. Gimble and Bruce A. Bunnell, date: 03/05/2017, view: 3720, Q&A: 0
Adipose-derived stromal/stem cells (ASCs) are multipotent cells that can be isolated from adipose tissue. Studies have shown that cells have the capacity to self-renew and differentiate into adipocyte, chondrocyte, myocyte, and osteoblast lineages. ...
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A Streamlined Method for the Preparation of Growth Factor-enriched Thermosensitive Hydrogels from Soft Tissue
Hydrogels are an ideal medium for the expansion of cells in three dimensions. The ability to induce cell expansion and differentiation in a controlled manner is a key goal in tissue engineering. Here we describe a detailed method for producing ...
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