Jose O'Brien Pontificia Universidad Catolica de Chile
1 protocol
Adam Idoine Carnegie Institution
2 protocols

Agnès Groisillier Sorbonne Universités, University Pierre and Marie Curie
2 protocols

Aksiniya Asenova University of California, Davis
10 protocols

Alexandros Alexandratos University of Athens
8 protocols

Arsalan Daudi
  • University of California
Research focus
  • Plant science
  • 1 Author merit


Ph.D. in Molecular Plant Biology, Rothamsted Research (Partnership with Monsanto and University of East Anglia), UK, 2007

Current position

Arsalan is an Executive Editor at Bio-protocol. Previously, he was a postdoctoral fellow in the Plant Pathology Department at the University of California, Davis. His research background is in plant-microbe interactions, plant molecular biology, microbiology and genetics.


  1. Bahar, O., Mordukhovich, G., Luu, D. D., Schwessinger, B., Daudi, A., Jehle, A. K., Felix, G. and Ronald, P. C. (2016). Bacterial outer membrane vesicles induce plant immune responses. Mol Plant Microbe Interact 29(5): 374-384.   
  2. Pruitt, R. N., Schwessinger, B., Joe, A., Thomas, N., Liu, F., Albert, M.,  Robinson, M. R., Chan, L. J. G., Luu, D. D., Chen, H.,  Bahar, O., Daudi, A., Vleesschauwer, D. D., Caddell, D., Zhang, W., Zhao, X., Li, X., Heazlewood, J. L., Ruan, D., Majumder, D., Chern, M., Kalbacher, H., Midha, S., Patil, P. B., Sonti, R. V., Petzold, C. J., Liu, C. C., Brodbelt, J. S., Felix, G. and Ronald, P. C. (2015). The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium. Science Advances 1(6): e1500245.
  3. Schwessinger, B., Bahar, O., Thomas, N., Holton, N., Nekrasov, V., Ruan, D., Canlas, P. E., Daudi, A., Petzold, C. J., Singan, V. R., Kuo, R., Chovatia, M., Daum, C., Heazlewood, J. L., Zipfel, C. and Ronald, P. C. (2015). Transgenic expression of the dicotyledonous pattern recognition receptor EFR in rice leads to ligand-dependent activation of defense responses. PLoS Pathog 11(3): e1004809.   
  4. Mammarella, N. D., Cheng, Z., Fu, Z. Q., Daudi, A., Bolwell, G. P., Dong, X. and Ausubel, F. M. (2015). Apoplastic peroxidases are required for salicylic acid-mediated defense against Pseudomonas syringae. Phytochemistry (112): 110-121.
  5. Chen, X., Zuo, S., Schwessinger, B., Chern, M., Canlas, P. E., Ruan, D., Zhou, X., Wang, J., Daudi, A., Petzold, C. J., Heazlewood, J. L. and Ronald, P. C. (2014). An XA21-associated kinase (OsSERK2) regulates immunity mediated by the XA21 and XA3 immune receptors. Mol Plant 7(5): 874-892.
  6. Bahar, O., Pruitt, R., Luu, D. D., Schwessinger, B., Daudi, A., Liu, F., Ruan, R., Fontaine-Bodin, L., Koebnik, R. and Ronald, P. C. (2014). The Xanthomonas Ax21 protein is processed by the general secretory system and is secreted in association with outer membrane vesicles. PeerJ 2: e242.
  7. Daudi, A. and O’Brien, J. A. (2012). Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves. Bio-protocol 2(18): e263.
  8. O’Brien, J. A., Daudi, A., Butt, V. S. and Bolwell, G. P. (2012). Reactive oxygen species and their role in plant defence and cell wall metabolism. Planta 236(3): 765-779.
  9. Daudi, A., Cheng, Z., O’Brien, J. A., Mammarella, N., Khan, S., Ausubel, F. M. and Bolwell, G. P. (2012). The apoplastic oxidative burst peroxidase in Arabidopsis is a major component of pattern-triggered immunity. Plant Cell 24(1): 275-287.
  10. O’Brien, J. A., Daudi, A., Finch, P., Butt, V. S., Whitelegge, J. P., Souda, P., Ausubel, F. M. and Bolwell, G. P. (2012). A peroxidase-dependent apoplastic oxidative burst in cultured Arabidopsis cells functions in MAMP-elicited defense. Plant Physiol 158(4): 2013-2027.
  11. Cook, C. M., Daudi, A., Millar, D. J., Bindschedler, L. V., Khan, S., Bolwell, G. P. and Devoto, A. (2012). Transcriptional changes related to secondary wall formation in xylem of transgenic lines of tobacco altered for lignin or xylan content which show improved saccharification. Phytochemistry 74: 79-89.
  12. Lowe, R. G., Allwood, J. W., Galster, A. M., Urban, M., Daudi, A., Canning, G., Ward, J. L., Beale, M. H. and Hammond-Kosack, K. E. (2010). A combined 1H nuclear magnetic resonance and electrospray ionization-mass spectrometry analysis to understand the basal metabolism of plant-pathogenic Fusarium spp. Mol Plant Microbe Interact 23(12): 1605-1618.
  13. Kavousi, B., Daudi, A., Cook, C. M., Joseleau, J. P., Ruel, K., Devoto, A., Bolwell, G. P. and Blee, K. A. (2010). Consequences of antisense down-regulation of a lignification-specific peroxidase on leaf and vascular tissue in tobacco lines demonstrating enhanced enzymic saccharification. Phytochemistry 71(5-6): 531-542.
  14. Nakagami, H., Sugiyama, N., Mochida, K., Daudi, A., Yoshida, Y., Toyoda, T., Tomita, M., Ishihama, Y. and Shirasu, K. (2010). Large-scale comparative phosphoproteomics identifies conserved phosphorylation sites in plants. Plant Physiol 153(3): 1161-1174.
  15. Bolwell, G. P. and Daudi, A. (2009). Reactive oxygen species in plant–pathogen interactions. Reactive oxygen species in plant signaling. Springer, 113-133. 
  16. Sugiyama, N., Nakagami, H., Mochida, K., Daudi, A., Tomita, M., Shirasu, K. and Ishihama, Y. (2008). Large-scale phosphorylation mapping reveals the extent of tyrosine phosphorylation in Arabidopsis. Mol Syst Biol 4:193.
  17. Hammond-Kosack, K., Urban, M., Baldwin, T., Daudi, A., Rudd, J., Keon, J., Lucas, J., Maguire, K., Kornyukhin, D. and Jing, H. (2004). Plant pathogens: how can molecular genetic information on plant pathogens assist in breeding disease resistant crops. 4th International Crop Science Congress. 
1 Protocol published
Detection of Hydrogen Peroxide by DAB Staining in Arabidopsis Leaves
Authors:  Arsalan Daudi and Jose A. O’Brien, date: 09/20/2012, view: 39948, Q&A: 17
In this protocol, the in situ detection of hydrogen peroxide (one of several reactive oxygen species) is described in mature Arabidopsis rosette leaves by staining with 3,3'-diaminobenzidine (DAB) using an adaptation of previous ...
19 Protocols reviewed
Metabolite Profiling of Mature Arabidopsis thaliana Seeds Using Gas Chromatography-Mass Spectrometry (GC-MS)
Authors:  Hagai Cohen, Ifat Matityahu and Rachel Amir, date: 11/05/2016, view: 2957, Q&A: 0
Metabolite profiling using gas chromatography-mass spectrometry (GC-MS) permits the annotation and quantification of a relatively wide variety of metabolites, covering a wide range of biochemical groups of metabolites. Lisec et al. (2006) ...
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Paper Roll Culture and Assessment of Maize Root Parameters
Authors:  Adel H. Abdel-Ghani, Darlene L. Sanchez, Bharath Kumar and Thomas Lubberstedt, date: 09/20/2016, view: 3364, Q&A: 0
Selection for genotypes with a vigorous root system could enhance the adaptation of maize under water and nutrient deficit soils. Although extensive genetic variation for root architecture has been reported (Kumar et al., 2012; Abdel-Ghani ...
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134 Protocols edited
MTT Assay for Cytotoxicity Assessment in Oryza sativa Root Tissue
Authors:  Snehalata Majumdar, Titir Guha and Rita Kundu, date: 11/20/2017, view: 609, Q&A: 0
Cytotoxicity of different compounds are commonly evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. This assay is mainly used to study cell viability in cell lines (Carmichael et al., 1987). In this ...
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Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method
Authors:  Yinggen Ke, Shugang Hui and Meng Yuan, date: 10/05/2017, view: 1272, Q&A: 0
Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of Xoo and ...
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