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Andrew Norton Durham University Business School, Durham University, UK
1 protocol

Yvette Coulson-Thomas Department of Biochemistry, Universidade Federal de São Paulo, Brazil
2 protocols

tarsis ferreira OPTIMVIA
1 protocol
Alka Mehra Institute of Genomics and Integrative Biology
19 protocols

Anca Savulescu University of Cape Town
13 protocols

Christopher Poon St. Vincent’s Institute of Medical Research
2 protocols

Dongsheng Jiang Comprehensive Pneumology Center, Helmholtz Zentrum München, Germany
2 protocols

Editor
Vivien J. Coulson-Thomas
  • Assistant professor, University of Houston
Research focus
  • Stem cell
  • 4 Author merit
4 Protocols published
Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth
Authors:  Yvette May Coulson-Thomas, Vivien Jane Coulson-Thomas and Andrew L. Norton, date: 04/05/2017, view: 2476, Q&A: 0
We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas et al., 2015) and are described here in more detail. In the case ...
3D Stroma Invasion Assay
Authors:  Yvette May Coulson-Thomas and Vivien Jane Coulson-Thomas, date: 03/20/2017, view: 2897, Q&A: 0
We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of ...
5 Protocols reviewed
In vitro Chondrogenic Hypertrophy Induction of Mesenchymal Stem Cells
Authors:  Sang Young Jeong, Miyoung Lee, Soo Jin Choi, Wonil Oh and Hong Bae Jeon, date: 12/05/2016, view: 4406, Q&A: 0
To investigate underlying mechanism of chondrogenic hypertrophy, we need proper in vitro hypertrophic model of mesenchymal stem cells (MSCs). This protocol describes our defined method for induction of in vitro chondrogenic ...
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Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures
Authors:  Xiurong Zhao, Liyan Zhang, Shun-Ming Ting and Jaroslaw Aronowski, date: 04/20/2016, view: 4662, Q&A: 0
Clearance of dead brain tissue including the dead neurons through phagocytosis is an endogenous function of microglia in the brain, which is critical for inflammation resolution after ischemic stroke or head trauma. By regulating the function or ...
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15 Protocols edited
Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
Authors:  Cindy L. Linn, Sarah E. Webster and Mark K. Webster, date: 11/05/2018, view: 78, Q&A: 0
Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate ...
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Using Stable Isotopes in Bone Marrow Derived Macrophage to Analyze Metabolism
Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently ...
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