Vivien J. Coulson-Thomas

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Andrew Norton Durham University Business School, Durham University, UK
1 protocol

Yvette Coulson-Thomas Department of Biochemistry, Universidade Federal de São Paulo, Brazil
2 protocols

tarsis ferreira OPTIMVIA
1 protocol

Alka Mehra Institute of Genomics and Integrative Biology
27 protocols

Anca Savulescu University of Cape Town
19 protocols

Christopher Poon St. Vincent’s Institute of Medical Research
2 protocols

Dongsheng Jiang Comprehensive Pneumology Center, Helmholtz Zentrum München, Germany
2 protocols

Editor

Vivien J. Coulson-Thomas

Assistant professor, University of Houston

Research focus

Stem cell

4 Author merit
Reviewer merit
Editor merit

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=coulson-+thomas+vj

4 Protocols published

Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth

Authors: Yvette May Coulson-Thomas, Vivien Jane Coulson-Thomas and Andrew L. Norton, date: 04/05/2017, view: 3958, Q&A: 0

We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas et al., 2015) and are described here in more detail. In the case ...

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3D Stroma Invasion Assay

Authors: Yvette May Coulson-Thomas and Vivien Jane Coulson-Thomas, date: 03/20/2017, view: 4828, Q&A: 0

We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of ...

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5 Protocols reviewed

In vitro Chondrogenic Hypertrophy Induction of Mesenchymal Stem Cells

Authors: Sang Young Jeong, Miyoung Lee, Soo Jin Choi, Wonil Oh and Hong Bae Jeon, date: 12/05/2016, view: 6669, Q&A: 0

To investigate underlying mechanism of chondrogenic hypertrophy, we need proper in vitro hypertrophic model of mesenchymal stem cells (MSCs). This protocol describes our defined method for induction of in vitro chondrogenic ...

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Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures

Authors: Xiurong Zhao, Liyan Zhang, Shun-Ming Ting and Jaroslaw Aronowski, date: 04/20/2016, view: 6606, Q&A: 0

Clearance of dead brain tissue including the dead neurons through phagocytosis is an endogenous function of microglia in the brain, which is critical for inflammation resolution after ischemic stroke or head trauma. By regulating the function or ...

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17 Protocols edited

A Robust, One-step FRET Assay for Human Heparanase

Authors: Jyothi C. Sistla and Umesh R. Desai, date: 09/05/2019, view: 433, Q&A: 0

Heparanase, an endo-β-D-glucuronidase, cleaves cell surface and extracellular matrix heparan sulfate (HS) chains at distinct sites and plays important biological roles including modulation of cell growth and metastasis. Although a number of ...

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Cell Microencapsulation and Cryopreservation with Low Molecular Weight Hyaluronan and Dimethyl Sulfoxide

Authors: H. Gurruchaga, L. Saenz del Burgo, G. Orive, R. M. Hernandez, J. Ciriza and J. L. Pedraz, date: 02/20/2019, view: 1423, Q&A: 0

Cryopreservation is commonly used for the storage of cells, tissues, organs or 3D cell-based products using ultra-low temperatures, which involves the immersion in liquid nitrogen for their long-term preservation. The cryopreservation of several ...

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