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A peer-reviewed protocol journal. No publication fee; no access fee.
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Andrew Norton
Durham University Business School, Durham University, UK
1 protocol
Yvette Coulson-Thomas
Department of Biochemistry, Universidade Federal de São Paulo, Brazil
2 protocols
tarsis ferreira
OPTIMVIA
1 protocol
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Alka Mehra
Institute of Genomics and Integrative Biology
19 protocols
Anca Savulescu
University of Cape Town
13 protocols
Christopher Poon
St. Vincent’s Institute of Medical Research
2 protocols
Dongsheng Jiang
Comprehensive Pneumology Center, Helmholtz Zentrum München, Germany
2 protocols
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Editor
Vivien J. Coulson-Thomas
Assistant professor, University of Houston
Research focus
Stem cell
Contributions
4
Author merit
Reviewer merit
Editor merit
Publications
https://www.ncbi.nlm.nih.gov/pubmed/?term=coulson-+thomas+vj
4 Protocols published
Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth
Authors:
Yvette May Coulson-Thomas
,
Vivien Jane Coulson-Thomas
and
Andrew L. Norton
,
date:
04/05/2017,
view:
2476,
Q&A:
0
We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas
et al
., 2015) and are described here in more detail. In the case ...
More >
3D Stroma Invasion Assay
Authors:
Yvette May Coulson-Thomas
and
Vivien Jane Coulson-Thomas
,
date:
03/20/2017,
view:
2897,
Q&A:
0
We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of ...
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5 Protocols reviewed
In vitro
Chondrogenic Hypertrophy Induction of Mesenchymal Stem Cells
Authors:
Sang Young Jeong
,
Miyoung Lee
,
Soo Jin Choi
,
Wonil Oh
and
Hong Bae Jeon
,
date:
12/05/2016,
view:
4406,
Q&A:
0
To investigate underlying mechanism of chondrogenic hypertrophy, we need proper
in vitro
hypertrophic model of mesenchymal stem cells (MSCs). This protocol describes our defined method for induction of
in vitro
chondrogenic ...
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Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures
Authors:
Xiurong Zhao
,
Liyan Zhang
,
Shun-Ming Ting
and
Jaroslaw Aronowski
,
date:
04/20/2016,
view:
4662,
Q&A:
0
Clearance of dead brain tissue including the dead neurons through phagocytosis is an endogenous function of microglia in the brain, which is critical for inflammation resolution after ischemic stroke or head trauma. By regulating the function or ...
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15 Protocols edited
Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
Authors:
Cindy L. Linn
,
Sarah E. Webster
and
Mark K. Webster
,
date:
11/05/2018,
view:
78,
Q&A:
0
Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate ...
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Using Stable Isotopes in Bone Marrow Derived Macrophage to Analyze Metabolism
Authors:
Chih-Wei Ko
,
Daniel Counihan
,
David DeSantis
,
Zach Sedor-Schiffhauer
,
Michelle Puchowicz
and
Colleen M Croniger
,
date:
09/05/2018,
view:
369,
Q&A:
0
Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently ...
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