Andrew Norton Durham University Business School, Durham University, UK
1 protocol

Yvette Coulson-Thomas Department of Biochemistry, Universidade Federal de São Paulo, Brazil
2 protocols

tarsis ferreira OPTIMVIA
1 protocol
Alka Mehra Institute of Genomics and Integrative Biology
45 protocols

Anca Savulescu University of Cape Town
24 protocols

Christopher Poon St. Vincent’s Institute of Medical Research
2 protocols

Dongsheng Jiang Institute of Regenerative Biology and Medicine, Helmholtz Zentrum München, Germany
3 protocols

Editor
Vivien J. Coulson-Thomas
  • Assistant professor, University of Houston
Research focus
  • Stem cell
  • 4 Author merit
4 Protocols published
Isolation and Analysis of Proteoglycans and Glycosaminoglycans from Archaeological Bones and Teeth
Authors:  Yvette May Coulson-Thomas, Vivien Jane Coulson-Thomas and Andrew L. Norton, date: 04/05/2017, view: 6712, Q&A: 0
We have developed methods for isolating proteoglycans and glycosaminoglycans from archaeological bones and teeth. These methods have been previously published (Coulson-Thomas et al., 2015) and are described here in more detail. In the case ...
3D Stroma Invasion Assay
Authors:  Yvette May Coulson-Thomas and Vivien Jane Coulson-Thomas, date: 03/20/2017, view: 7622, Q&A: 0
We have developed a 3D co-culture system composed of fibroblasts and colorectal cancer cells that enables us to study the desmoplastic reaction. This method also enables us to study the influence of the desmoplastic reaction on the migration of ...
5 Protocols reviewed
In vitro Chondrogenic Hypertrophy Induction of Mesenchymal Stem Cells
Authors:  Sang Young Jeong, Miyoung Lee, Soo Jin Choi, Wonil Oh and Hong Bae Jeon, date: 12/05/2016, view: 11187, Q&A: 1
To investigate underlying mechanism of chondrogenic hypertrophy, we need proper in vitro hypertrophic model of mesenchymal stem cells (MSCs). This protocol describes our defined method for induction of in vitro chondrogenic ...
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Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures
Authors:  Xiurong Zhao, Liyan Zhang, Shun-Ming Ting and Jaroslaw Aronowski, date: 04/20/2016, view: 9779, Q&A: 0
Clearance of dead brain tissue including the dead neurons through phagocytosis is an endogenous function of microglia in the brain, which is critical for inflammation resolution after ischemic stroke or head trauma. By regulating the function or ...
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21 Protocols edited
Quantitative Live Confocal Imaging in Aquilegia Floral Meristems
Authors:  Ya Min, Stephanie J. Conway and Elena M. Kramer, date: 06/20/2022, view: 989, Q&A: 0

In this study, we present a detailed protocol for live imaging and quantitative analysis of floral meristem development in Aquilegia coerulea, a member of the buttercup family (Ranunculaceae). Using confocal microscopy and the image analysis

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Isolation of Liver Mononuclear Cells from a Cholestatic Mice for Single Cell or Single Nuclei Sequencing
Authors:  Ramesh Kudira, Srikar Pasula, Shwetha Kapil and Alexander Miethke, date: 05/05/2022, view: 183, Q&A: 0

Cholestasis develops as a consequence of impaired bile formation, secretion, and flow from the liver to the duodenum, causing retention of bile in the body. Cholestasis is classified as intra or extrahepatic cholangitis, primary biliary cholangitis

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