Suchetana Mukhopadhyay Biology, Indiana University, USA, USA,
1 protocol

Joseph Wang Department of Biology, Indiana University, USA, USA,
1 protocol

Jolene Ramsey Department of Biology, Indiana University, USA, USA,
1 protocol

Brian Bothner Department of Chemistry and Biochemistry, Montana State University, USA
2 protocols
Balaji Olety Amaranath University of Massachusetts medical School
2 protocols

Balasubramanian Venkatakrishnan Syngene International Limited
3 protocols

Daniela Boehm Gladstone Institute of Virology and Immunology
3 protocols

David Paul MRC Laboratory of Molecular Biology
22 protocols

Editor
Vamseedhar Rayaprolu
  • Research scientist, La Jolla Institute for Allergy and Immunology
Research focus
  • Biophysics
  • Structural virology with a focus on viral assembly, functionalization and biology
    Membrane biology focused on stoichiometry and function
  • 2 Author merit

Education

PhD in Biochemistry, Montana State University, 2013

Lab information

https://www.lji.org/faculty-research/labs/saphire/#overview

Publications

2 Protocols published
Alphavirus Purification Using Low-speed Spin Centrifugation
Authors:  Vamseedhar Rayaprolu, Jolene Ramsey, Joseph Che-Yen Wang and Suchetana Mukhopadhyay, date: 03/20/2018, view: 4052, Q&A: 2
Chemical and sedimentation procedures are used to purify virus particles. While these approaches are successful for wild-type viruses, they are often not feasible for purifying mutant viruses with assembly defects. We combined two published methods ...
Fluorometric Estimation of Viral Thermal Stability
Authors:  Vamseedhar Rayaprolu, Shannon Kruse, Ravi Kant, Navid Movahed, Dewey Brooke and Brian Bothner, date: 08/05/2014, view: 7260, Q&A: 1
Differential Scanning Fluorimetry (DSF) is a rapid, economical, and a straightforward technique for estimating the thermal stability of proteins. The principle involves the binding of a fluorescent dye to thermally exposed hydrophobic pockets of a ...
8 Protocols reviewed
In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
Authors:  Grant Schauer, Jeff Finkelstein and Mike O’Donnell, date: 09/20/2017, view: 5134, Q&A: 0
The eukaryotic replisome is a multiprotein complex that duplicates DNA. The replisome is sculpted to couple continuous leading strand synthesis with discontinuous lagging strand synthesis, primarily carried out by DNA polymerases ε and δ, ...
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Modification of 3’ Terminal Ends of DNA and RNA Using DNA Polymerase θ Terminal Transferase Activity
Authors:  Trung M. Hoang, Tatiana Kent and Richard T. Pomerantz, date: 06/20/2017, view: 4854, Q&A: 0
DNA polymerase θ (Polθ) is a promiscuous enzyme that is essential for the error-prone DNA double-strand break (DSB) repair pathway called alternative end-joining (alt-EJ). During this form of DSB repair, Polθ performs terminal transferase activity ...
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16 Protocols edited
Image-based Quantification of Direct Cell-to-cell Transmission of Bovine Viral Diarrhea Virus
Authors:  Fernando Merwaiss and Diego E. Alvarez, date: 08/05/2019, view: 532, Q&A: 0
Different viruses rely on direct cell-to-cell transmission to propagate infection within the infected host. Measuring this mode of transmission in cultured cells is often complicated by the contribution of cell free viruses to spread, and the ...
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Analysis of Functional Virus-generated PAMP RNAs Using IFNα/β ELISA Assay
Authors:  Margit Mutso, Xiang Liu, Andres Merits and Suresh Mahalingam, date: 06/20/2019, view: 689, Q&A: 0
Virus-generated PAMP RNAs are key factors that activate host immune response. The PAMP RNAs are therefore usually closely related with viral disease pathogenesis. Quantitative real time PCR is a conventional method to assess RNA. However, it cannot ...
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