Pan-Chyr Yang Institute of Biomedical Sciences, Academia Sinica, Taiwan
3 protocols

Hsueh-Fen Juan Department of Life Science, Institute of Molecular and Cellular Biology, National Taiwan University, Taiwan
2 protocols

Yi-Ching Wang Department of Pharmacology, National Cheng Kung University, Taiwan
2 protocols

Tsui-Chin Huang Institute of Molecular and Cellular Biology, National Taiwan University, Taiwan
2 protocols
Guillermo Gomez University of South Australia
5 protocols

HongLok Lung University of Hong Kong
57 protocols

Jia Li SRI International
45 protocols

Lee-Hwa Tai Universite de Sherbrooke
20 protocols

Hsin-Yi Chang
  • Faculty, Kyoto University
Research focus
  • Proteomics
  • 2 Author merit
2 Protocols published
Flow Cytometric Detection of Reactive Oxygen Species
Reactive oxygen species (ROS) are molecules containing hydroxyl radicals or peroxides with unpaired electrons. In healthy aerobic cells, ROS are produced naturally as a byproduct of oxidative phosphorylation, oxidoreductase enzymes, or metal ...
Flow Cytometric Detection of Mitochondrial Membrane Potential
Mitochondrial membrane potential (Δψm) is an important parameter of mitochondrial function and an indicator of cell health. Depletion of Δψm suggests the loss of mitochondrial membrane integrity reflecting the initiation of the proapoptotic signal. ...
7 Protocols reviewed
Non-radioactive LATS in vitro Kinase Assay
Authors:  Audrey W. Hong and Kun-Liang Guan, date: 07/20/2017, view: 2709, Q&A: 1
This protocol describes a method to directly measure LATS activity by an in vitro kinase assay using YAP as a substrate.
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Ca2+ Measurements in Mammalian Cells with Aequorin-based Probes
Authors:  Anna Tosatto, Rosario Rizzuto and Cristina Mammucari, date: 03/05/2017, view: 2504, Q&A: 0
Aequorin is a Ca2+ sensitive photoprotein suitable to measure intracellular Ca2+ transients in mammalian cells. Thanks to recombinant cDNAs expression, aequorin can be specifically targeted to various subcellular compartments, ...
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