I have the same questions, when I do stimulation of mouse splenocytes with 500 ng/ml ionomycin, 500 ng/ml PMA and Golgistop at the same time fro about 4 hours, the cells looked like they all die or lyse. So I have to stop at this step. Did I add too much PMA? but according to BD protocol, IL-17 stimulation need this condition. Should I add Golgistop right after stimulation?
8/30/2016 7:50:47 AM Reply