Manuel Lopez
  • Bio-protocol
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Optical Clearing Using SeeDB
Authors:  Meng-Tsen Ke, Satoshi Fujimoto and Takeshi Imai, date: 02/05/2014, view: 15430, Q&A: 1
We describe a water-based optical clearing agent, SeeDB (See Deep Brain), which clears fixed brain samples in a few days without quenching many types of fluorescent dyes, including fluorescent proteins and lipophilic neuronal tracers. SeeDB is a saturated solution of fructose (80.2% w/w) in water with 0.5% α-thioglycerol. In standard SeeDB optical ...