Takeshi Imai —BIO-PROTOCOL

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Meng-Tsen Ke Laboratory for Sensory Circuit Formation, RIKEN Center for Developmental Biology, Japan
3 protocols

Satoshi Fujimoto Kyushu University
1 protocol

Takeshi Imai

Professor, Graduate School of Medical Sciences, Kyushu University, Japan

Research focus

Neuroscience and Developmental Biology

3 Author merit
Reviewer merit
Editor merit

Education

Ph.D. in Biophysics and Biochemistry, The University of Tokyo, Japan, 2006

Current position

Professor, Graduate School of Medical Sciences, Kyushu University, Japan

Publications (selected)

Google Scholar: https://scholar.google.co.jp/citations?user=VPwm0QUAAAAJ&hl=ja
Iwata, R., Kiyonari, H. and Imai, T. (2017). Mechanosensory-Based Phase Coding of Odor Identity in the Olfactory Bulb. Neuron 96(5): 1139-1152 e1137.
Ke, M. T., Nakai, Y., Fujimoto, S., Takayama, R., Yoshida, S., Kitajima, T. S., Sato, M. and Imai, T. (2016). Super-Resolution Mapping of Neuronal Circuitry With an Index-Optimized Clearing Agent. Cell Rep 14(11): 2718-2732.
Nakashima, A.*, Takeuchi, H.*, Imai, T.*, Saito, H., Kiyonari, H., Abe, T., Chen, M., Weinstein, L. S., Yu, C. R., Storm, D. R., Nishizumi, H. and Sakano, H. (2013). Agonist-independent GPCR activity regulates anterior-posterior targeting of olfactory sensory neurons. Cell 154(6): 1314-1325. (*Equally contributed)
Ke, M. T., Fujimoto, S. and Imai, T. (2013). SeeDB: a simple and morphology-preserving optical clearing agent for neuronal circuit reconstruction. Nat Neurosci 16(8): 1154-1161.
Imai, T., Sakano, H. and Vosshall, L. B. (2010). Topographic mapping--the olfactory system. Cold Spring Harb Perspect Biol 2(8): a001776. (Review)
Imai, T., Yamazaki, T., Kobayakawa, R., Kobayakawa, K., Abe, T., Suzuki, M. and Sakano, H. (2009). Pre-target axon sorting establishes the neural map topography. Science 325(5940): 585-590.
Imai, T., Suzuki, M. and Sakano, H. (2006). Odorant receptor-derived cAMP signals direct axonal targeting. Science 314(5799): 657-661.

3 Protocols published

Correction: Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2

Authors: Meng-Tsen Ke and Takeshi Imai, date: 11/05/2018, view: 763, Q&A: 0

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Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2

Authors: Meng-Tsen Ke and Takeshi Imai, date: 10/20/2018, view: 3555, Q&A: 1

Tissue clearing techniques are useful for large-scale three-dimensional fluorescence imaging of thick tissues. However, high-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical ...

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Nathalie Agudelo

Institute of Science and Technology (IST) Austria

Dear authors,
I would kindly like to ask you the weight percentage to prepare the SeeDB2G mounting solution from Histodenz powder (Omnipaque 350 solution). According to your previous publication, it should be 56.2% w/w iohexol in Tris-EDTA buffer, however, preparing the solution as 10g Histodenz in 7.5 mL Tris-EDTA gives ~57.1% w/w. Additionally, you mention that low concentrations of Triton X-100 can also work for thin brain slices (instead of using 2% saponin), what range is recommended in this case to still preserve cell morphology?
Thank you very much for your attention.

12/2/2018 8:35:01 AM Reply

Takeshi Imai

Graduate School of Medical Sciences, Kyushu University, Japan

To prepare SeeDB2G, dissolve 10g Histodenz in 7.5 mL Tris-EDTA (~57.1% w/w) as described in Recipes. The refractive index will be exactly the same as that of Omnipaque350 (1.4662).

In most applications, there will be no need for further refinement. Please note, however, that the immersion liquid for some glycerol lenses may have a slightly lower refractive index. For example, 92% w/w Omnipaque350 matches Type G immersion liquid.

As for Triton X-100, we recommend 0.1-0.5% to minimize morphological damages. 2% is too high for Triton X-100.

To further minimize morphological damages, we also suggest more gradual clearing with intermediate concentration steps.

2018-12-06 22:14:49

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