Jerry Shay Department of Cell Biology, University of Texas Southwestern Medical Center, USA
3 protocols
Alba Blesa Universidad Francisco de Vitoria
26 protocols

Alka Mehra Institute of Genomics and Integrative Biology
40 protocols

Amriti Lulla UT MD Anderson Cancer Center
6 protocols

Ana Almeida University College Cork
8 protocols

Editor
ilgen Mender
  • Post-Doc, University of Texas Southwestern Medical Center
Research focus
  • Cancer biology
  • Immuno-oncology
  • 3 Author merit
3 Protocols published
Telomere Restriction Fragment (TRF) Analysis
Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 17173, Q&A: 0
While telomerase is expressed in ~90% of primary human tumors, most somatic tissue cells except transiently proliferating stem-like cells do not have detectable telomerase activity (Shay and Wright, 1996; Shay and Wright, 2001). Telomeres ...
Telomerase Repeated Amplification Protocol (TRAP)
Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 16426, Q&A: 1
Telomeres are found at the end of eukaryotic linear chromosomes, and proteins that bind to telomeres protect DNA from being recognized as double-strand breaks thus preventing end-to-end fusions (Griffith et al., 1999). However, due to the ...
6 Protocols reviewed
Single-molecule Fluorescence Technique to Monitor the Co-transcriptional Formation of G-quadruplex and R-loop Structures
Authors:  Gunhyoung Lim and Sungchul Hohng, date: 07/05/2021, view: 1337, Q&A: 0

G-quadruplexes (GQ) and R-loops are non-canonical nucleic acid structures related to gene regulation and genome instability that can be formed during transcription; however, their formation mechanisms remain elusive. To address this question, we

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Isolation, Purification and Characterization of Exosomes from Fibroblast Cultures of Skeletal Muscle
Authors:  Diantha van de Vlekkert, Xiaohui Qiu, Ida Annunziata and Alessandra d'Azzo, date: 04/05/2020, view: 2734, Q&A: 0
Exosomes are dynamic nanovesicles secreted by virtually all cells and are present in all biological fluids. Given their highly heterogeneous content exosomes have been implicated in many physiological and pathological processes that they exert by ...
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