Karen Buttigieg Faculty of Medicine, Imperial College London, UK
1 protocol

Stephen Laidlaw Section of Virology, Faculty of Medicine, Imperial College London, UK
1 protocol

Michael A. Skinner
  • Section of Virology, Imperial College London, UK
Research focus
  • Microbiology
  • 2 Author merit


Ph.D. in Biochemistry, University of Leicester

Current position

Senior Lecturer, Head of ‘Vaccine Vector’ group, Section of Virology, Faculty of Medicine, Imperial College London

Publications (since 2000)

Refereed publications

  1. Buttigieg, K., Laidlaw, S. M., Ross, C., Davies, M., Goodbourn, S. and Skinner, M. A. (2013). Genetic screen of a library of chimeric poxviruses identifies an ankyrin repeat protein involved in resistance to the avian type I interferon response. J Virol 87(9): 5028-5040.
  2. aidlaw, S. M., Robey, R., Davies, M., Giotis, E. S., Ross, C., Buttigieg, K., Goodbourn, S. and Skinner, M. A. (2013). Genetic screen of a mutant poxvirus library identifies an ankyrin repeat protein involved in blocking induction of avian type I interferon. J Virol 87(9): 5041-5052.
  3. Jeshtadi, A., Burgos, P., Stubbs, C. D., Parker, A. W., King, L. A., Skinner, M. A. and Botchway, S. W. (2010). Interaction of poxvirus intracellular mature virion proteins with the TPR domain of kinesin light chain in live infected cells revealed by two-photon-induced fluorescence resonance energy transfer fluorescence lifetime imaging microscopy. J Virol 84(24): 12886-12894.
  4. Bridge, S. H., Sharpe, S. A., Dennis, M. J., Dowall, S. D., Getty, B., Anson, D. S., Skinner, M. A., Stewart, J. P. and Blanchard, T. J. (2011). Heterologous prime-boost-boost immunisation of Chinese cynomolgus macaques using DNA and recombinant poxvirus vectors expressing HIV-1 virus-like particles. Virol J 8: 429.
  5. Bridge, S., Sharpe, S., Dennis, M., Dowall, S., Getty, B., Skinner, M., Hahn, B., Stewart, J. and Blanchard, T. (2010). HIV-neutralising response to recombinant, cross-clade, adjuvanted, virus-like particle-forming vaccine candidates. HIV MEDICINE, WILEY-BLACKWELL PUBLISHING, INC COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA.
  6. Guzman, E., Taylor, G., Charleston, B., Skinner, M. A. and Ellis, S. A. (2008). An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV. J Gen Virol 89(Pt 3): 667-675.
  7. Chaudhry, Y., Skinner, M. A. and Goodfellow, I. G. (2007). Recovery of genetically defined murine norovirus in tissue culture by using a fowlpox virus expressing T7 RNA polymerase. J Gen Virol 88(Pt 8): 2091-2100.
  8. Childs, K., Stock, N., Ross, C., Andrejeva, J., Hilton, L., Skinner, M., Randall, R. and Goodbourn, S. (2007). mda-5, but not RIG-I, is a common target for paramyxovirus V proteins. Virology 359(1): 190-200.
  9. Jarmin, S. A., Manvell, R., Gough, R. E., Laidlaw, S. M. and Skinner, M. A. (2006). Retention of 1.2 kbp of 'novel' genomic sequence in two European field isolates and some vaccine strains of Fowlpox virus extends open reading frame fpv241. J Gen Virol 87(Pt 12): 3545-3549.
  10. Imoukhuede, E. B., Berthoud, T., Milligan, P., Bojang, K., Ismaili, J., Keating, S., Nwakanma, D., Keita, S., Njie, F., Sowe, M., Todryk, S., Laidlaw, S. M., Skinner, M. A., Lang, T., Gilbert, S., Greenwood, B. M. and Hill, A. V. (2006). Safety and immunogenicity of the malaria candidate vaccines FP9 CS and MVA CS in adult Gambian men. Vaccine 24(42-43): 6526-6533.
  11. Jarmin, S., Manvell, R., Gough, R. E., Laidlaw, S. M. and Skinner, M. A. (2006). Avipoxvirus phylogenetics: identification of a PCR length polymorphism that discriminates between the two major clades. J Gen Virol 87(Pt 8): 2191-2201.
  12. Cottingham, M. G., van Maurik, A., Zago, M., Newton, A. T., Anderson, R. J., Howard, M. K., Schneider, J. and Skinner, M. A. (2006). Different levels of immunogenicity of two strains of Fowlpox virus as recombinant vaccine vectors eliciting T-cell responses in heterologous prime-boost vaccination strategies. Clin Vaccine Immunol 13(7): 747-757.
  13. Webster, D. P., Dunachie, S., Vuola, J. M., Berthoud, T., Keating, S., Laidlaw, S. M., McConkey, S. J., Poulton, I., Andrews, L., Andersen, R. F., Bejon, P., Butcher, G., Sinden, R., Skinner, M. A., Gilbert, S. C. and Hill, A. V. (2005). Enhanced T cell-mediated protection against malaria in human challenges by using the recombinant poxviruses FP9 and modified vaccinia virus Ankara. Proc Natl Acad Sci U S A 102(13): 4836-4841.
  14. Skinner, M. A., Laidlaw, S. M., Eldaghayes, I., Kaiser, P. and Cottingham, M. G. (2005). Fowlpox virus as a recombinant vaccine vector for use in mammals and poultry. Expert Rev Vaccines 4(1): 63-76.
  15. Jeshtadi, A., Henriquet, G., Laidlaw, S. M., Hot, D., Zhang, Y. and Skinner, M. A. (2005). In vitro expression and analysis of secreted fowlpox virus CC chemokine-like proteins Fpv060, Fpv061, Fpv116 and Fpv121. Arch Virol 150(9): 1745-1762.
  16. Moorthy, V. S., Imoukhuede, E. B., Keating, S., Pinder, M., Webster, D., Skinner, M. A., Gilbert, S. C., Walraven, G. and Hill, A. V. (2004). Phase 1 evaluation of 3 highly immunogenic prime-boost regimens, including a 12-month reboosting vaccination, for malaria vaccination in Gambian men. J Infect Dis 189(12): 2213-2219.
  17. Anderson, R. J., Hannan, C. M., Gilbert, S. C., Laidlaw, S. M., Sheu, E. G., Korten, S., Sinden, R., Butcher, G. A., Skinner, M. A. and Hill, A. V. (2004). Enhanced CD8+ T cell immune responses and protection elicited against Plasmodium berghei malaria by prime boost immunization regimens using a novel attenuated fowlpox virus. J Immunol 172(5): 3094-3100.
  18. Laidlaw, S. M. and Skinner, M. A. (2004). Comparison of the genome sequence of FP9, an attenuated, tissue culture-adapted European strain of Fowlpox virus, with those of virulent American and European viruses. J Gen Virol 85(Pt 2): 305-322.
  19. Boulanger, D., Green, P., Jones, B., Henriquet, G., Hunt, L. G., Laidlaw, S. M., Monaghan, P. and Skinner, M. A. (2002). Identification and characterization of three immunodominant structural proteins of fowlpox virus. J Virol 76(19): 9844-9855.
  20. Vazquez Blomquist, D., Green, P., Laidlaw, S. M., Skinner, M. A., Borrow, P. and Duarte, C. A. (2002). Induction of a strong HIV-specific CD8+ T cell response in mice using a fowlpox virus vector expressing an HIV-1 multi-CTL-epitope polypeptide. Viral Immunol 15(2): 337-356.
  21. Kataria, R. S., Tiwari, A. K., Butchaiah, G., Kataria, J. M. and Skinner, M. A. (2001). Sequence analysis of the VP2 gene hypervariable region of infectious bursal disease viruses from India. Avian Pathol 30(5): 501-507.
  22. Phenix, K. V., Wark, K., Luke, C. J., Skinner, M. A., Smyth, J. A., Mawhinney, K. A. and Todd, D. (2001). Recombinant Semliki Forest virus vector exhibits potential for avian virus vaccine development. Vaccine 19(23-24): 3116-3123.
  23. Brown, M., Zhang, Y., Dermine, S., de Wynter, E. A., Hart, C., Kitchener, H., Stern, P. L., Skinner, M. A. and Stacey, S. N. (2000). Dendritic cells infected with recombinant fowlpox virus vectors are potent and long-acting stimulators of transgene-specific class I restricted T lymphocyte activity. Gene Ther 7(19): 1680-1689.
  24. Das, S. C., Baron, M. D., Skinner, M. A. and Barrett, T. (2000). Improved technique for transient expression and negative strand virus rescue using fowlpox T7 recombinant virus in mammalian cells. J Virol Methods 89(1-2): 119-127.
  25. Boulanger, D., Smith, T. and Skinner, M. A. (2000). Morphogenesis and release of fowlpox virus. J Gen Virol 81(Pt 3): 675-687.

Non-refereend publications-books

  1. SKINNER, M. A. and LAIDLAW, S. M. (2012). Recombinant Avipoxviruses. In: Morrow, J. H., Schmidt, C., Sheikh, N. and Davies, H. (eds). Vaccinology: Principles and Practice. Wiley-Blackwell.
  2. SKINNER, M. A., BULLER, R. M., DAMON, I. K., LEFKOWIZ, E. J., MCFADDEN, G., MCINNES, C. J., MERCER, A. A. and MOYER, R. W. (2011). Family: Poxviridae. In: King, A. M., Lefkowitz, E., Adams, M. J. and Carstens, E. B. (eds). Virus Taxonomy - Ninth Report of the International Committee on Taxonomy of Viruses. Elsevier.
  3. SKINNER, M. A. (2008). Fowlpox virus and other avipoxviruses. In: Mahy, B. W. J. and Van, R. M. H. V. (eds). Encyclopedia of Virology. Third Ed. Academic Press.
  4. SMITH, G. L., BEARD, P. and SKINNER, M. A. (2008). Poxviruses. In: Mahy, B. W. J. and Van, R. M. H. V. (eds). Encyclopedia of Virology. Third Ed. Academic Press.
  5. SCHAT, K. A. and SKINNER, M. A. (2008). In: Davison, F., Kaspers, B. and Schat, K. A. (eds). Avian Immunology. Elsevier, 301-324.
  6. SKINNER, M. A. (2007). Avian Immunosuppressive Diseases and Immune Evasion. In: Pattison, M., McMullin, P., Bradbury, J. and Alexander, D. (eds). Poultry Diseases. 6th Ed. Elsevier.
2 Protocols published
Construction of FWPV Chimaeric MVA
Authors:  Karen R. Buttigieg and Michael A. Skinner, date: 01/05/2015, view: 6955, Q&A: 1
Construction of chimaeric MVA is a useful tool with which to study gene function of related viruses. The protocol given here describes MVA chimaeras containing genes from Fowlpox virus (FWPV), although this can be applied to DNA derived from other ...
Construction of Deletion-knockout Mutant Fowlpox Virus (FWPV)
Authors:  Stephen M. Laidlaw and Michael A. Skinner, date: 05/20/2014, view: 6546, Q&A: 0
The construction of deletion-knockout poxviruses is a useful approach to determining the function of specific virus genes. This protocol is an adaptation of the transient dominant knockout selection protocol published by Falkner and Moss (1990) for ...
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