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A peer-reviewed protocol journal. No publication fee; no access fee.
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Smita Nair
Center for Cancer Research, National Cancer Institute, USA
1 protocol
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Adam Idoine
Carnegie Institution
12 protocols
Adler Dillman
Nematology, UC Riverside, USA
11 protocols
Alka Mehra
Institute of Genomics and Integrative Biology
21 protocols
Amey Redkar
Department of Genetics, University of Cordoba
12 protocols
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Reviewer
Smita Nair
Research scientist, Indiana University
Research focus
Biochemistry
Molecular and cellular biology of Hepatitis B Virus
Contributions
1
Author merit
Reviewer merit
Editor merit
Education
PhD, Indian Institute of Science, 2010
Lab information
Adam Zlotnick Lab
1 Protocol published
In vitro
Assay for Cytidine Deaminase Activity of APOBEC3 Protein
Authors:
Smita Nair
and
Alan Rein
,
date:
10/20/2014,
view:
7141,
Q&A:
1
Cytidine deaminases are enzymes that catalyze the removal of an amino group from cytidine, forming uridine. APOBEC3 (ApolipoproteinB mRNA editing enzyme, catalytic polypeptide like) proteins are cytidine deaminases that deaminate cytidines in ...
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Deborah Fong
National University of Singapore
There was no deaminase activity detected, even for the positive control
12/16/2018 2:35:28 AM
Reply
14 Protocols reviewed
Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation
Authors:
Tyler Milston Renner
,
Kasandra Bélanger
and
Marc-André Langlois
,
date:
09/05/2018,
view:
605,
Q&A:
0
There exists a wide variety of techniques to isolate and purify viral particles from cell culture supernatants. However, these techniques vary greatly in ease of use, purity, yield and impact on viral structural integrity. Most importantly, it is ...
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Long-term
in vitro
Culture of
Cryptosporidium parvum
Authors:
Nigel Yarlett
and
Mary Morada
,
date:
08/05/2018,
view:
872,
Q&A:
0
Continuous
in vitro
growth of
Cryptosporidium parvum
has proved difficult and conventional
in vitro
culture techniques result in short-term (2-5 days) growth of the parasite resulting in thin-walled oocysts that fail to ...
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