Scott A Walper
  • Research scientist, Naval Research Laboratory
Research focus
  • Biochemistry
  • Synthetic biology
    Biomaterials development
    Cell-free systems
  • 0 Author merit


PhD, The University of Southern Mississippi, 2010

Lab information

Scott A. Walper Laboratory


1. Blum, SM; Lee, MS; Mgboji, GE; Funk, VL; Beabout, K; Harbaugh, SV; Roth, PA; Liem, AT; Miklos, AE, Emanuel, PA; Walper, SA; Chavez, JL, Lux, MW. Impact of Porous Matrices and Concentration by Lyophilization on Cell-Free Expression. ACS Synth Biol. 2021,
2. Dean, SN and Walper, SA. Variational Autoencoder for Generation of Antimicrobial Peptides. ACS Omega. 2020, 5(33): p 20746-20754.
3. Yuen, JD; Shriver-Lake, LC; Walper, SA; Zabetakis, D; Breger, JC; Stenger, DA. Microbial Nanocellulose Printed Circuit Boards for Medical Sensing. Sensors. 2020, 20(7): p 2047.
4. Dean SN, Rimmer MA, Turner KB, Phillips DA, Caruana JC, Hervey WJ, Walper, SA. Lactobacillus acidophilus Membrane Vesicles as a Vehicle of Bacteriocin Delivery. Front Microbiol. 2020, 11 (710).
5. Caruana, JC, Walper, SA. Bacterial Membrane Vesicles as Mediators of Microbe - Microbe and Microbe - Host Community Interactions. Front Microbiol. 2020, 11 (432).
6. Ellis, GA, Dean, SN, Walper, SA; Medintz, IL. Quantum Dots and Gold Nanoparticles As Scaffolds for Enzymatic Enhancement: Recent Advances and the Influence of Nanoparticle Size. Catalysts. 2020: 10(1): p 83-117.
7. Ellis, GA; Klein, WP; Lasarte-Aragones, G; Thakue, M; Walper, SA; Medintz, IL. Artificial Multienzyme Scaffolds: Pursuing in Vitro Substrate Channeling with an Overview of Current Progress. ACS Catalysis. 2019, 9: 10812-10869.
8. Cole, SD; Beabout, K; Turner, KB; Smith, ZK; Funk, VL; Harbaugh, SV; Liem, AT; Roth, PA; Grier, BA; Emanuel, PA; Walper, SA; Chavez, JL; Lux, MW. Quantification of Interlaboratory Cell-Free Protein Synthesis Variability. .ACS Synth Biol. 2019, 8(9): p 2080-2091.
9. Thakur, M; Medintz, IL; Walper, SA. Enzymatic Bioremediation of Organophosphate Compounds – Progress and Remaining Challenges. Front Bioeng Biotechnol. 2019, 7 (289).
10. Klein, WP; Thomsen, RP; Turner, KB; Walper, SA; Vranish, J; Kjems, J; Ancona, MG; Medintz, IL. Enhanced Catalysis from Multienzyme Cascades Assembled on a DNA Origami Triangle. ACS Nano. 2019, 13: 13677-13689.
11. Klein, WP; Diaz, SA, Chiriboga, M; Walper, SA, Medintz, IL. Dendrimeric DNA-based Nanoscaffolded BRET-FRET Optical Encryption Keys. ACS Appl Nano Mater. 2019, 2(12): p 7459.
12. Spangler, JR; Caruana, JC; Phillips, DA; Walper, SA. Broad Range Shuttle Vector Construction and Promoter Evaluation for the Use of Lactobacillus plantarum WCFS1 as a Microbial Engineering Platform. Synthetic Biology. 2019, 4(1): ysz012.
13. Breger, JC; Oh, E; Susumu, K; Klein, W; Walper, SA; Ancona, MG; Medintz, IL. Nanoparticle Size Influences Localized Enzymatic Enhancement – A Case Study with Phosphotriesterase. Bioconjugate Chemistry. 2019, 30(7): p 2060-2074.
14. Spangler, JR; Dean, SN; Leary, DH; Walper, SA. Response of Lactobacillus plantarum WCFS1 to the Gram-Negative Pathogen-Associated Quorum Sensing Molecule N-3-Oxododecanoyl Homoserine Lactone. Frontiers in Microbiology. 2019, 10: 715.
15. Dean, SN; Leary, DH; Sullivan, CJ; Oh, E; Walper, SA. Isolation and Characterization of Lactobacillus-Derived Membrane Vesicles. Scientific Reports. 2019, 9: p 877.
16. Anderson, GP; Shriver-Lake, LC; Walper, SA; Ashford, L; Zabetakis, D; Liu, JL; Breger, JC; Brozozog-Lee, A; Goldman, ER. Genetic Fusion of an Anti-BclA Single-Domain Antibody with Beta Galactosidase. Antibodies. 2018, 7(36): p 36-48.
17. Walper, SA; Lasarte-Aragones, G; Sapsford, KE; Brown III, CW; Rowland, CE; Breger, JC; Medintz, IL. Detecting Biothreat Agents: from Current Diagnostics to Developing Sensor Technologies. ACS Sensors. 2018, 3(10): p 1894-2024.
18. Vranish, JN; Ancona, MG; Oh, E; Susumu, K; Aragones, GL; Breger, JC; Walper, SA; Medintz, IL. Enhancing Coupled Enzymatic Activity by Colocalization of Nanoparticle Surfaces: Kinetic Evidence for Directed Channeling Intermediates. ACS Nano. 2018. 12(8), p 7911-7926.
19. Samanta, A; Breger, JC; Susumu, K; Oh, E; Walper, SA; Bassin, N; Medintz, IL. DNA-Nanoparticle Composites Synergistically Enhance Organophosphate Hydrolase Enzymatic Activity. ACS Applied Nano Materials 2018. 1 (7), p 3091-3097.
20. Field, LD; Walper, SA; Susumu, K; Lasarte-Aragones, G; Oh, E; Medintz, IL; Delehanty, JB. A Quantum Dot-Protein Bioconjugate That Provides for Extracellular Control of Intracellular Drug Release. ACS Bioconjugate Chemistry. 2018, 29 (7), p 2455-2467.
21. Alves, NJ; Moore, M; Johnson, BJ; Dean, SN; Turner, KB; Medintz, IL; Walper, SA. Environmental Decontamination of a Chemical Warfare Simulant Utilizing a Membrane Vesicle-Encapsulated Phosphotriesterase. ACS Applied Materials & Interface. 2018, 10 (18), p 15712-15719.
22. Hondred, J; Breger, J; Alves, NJ; Trammell, S; Walper, SA; Medintz, IL; Claussen, J. Printed Graphene Electrochemical Biosensors Fabricated by Inkjet Maskless Lithography for Rapid and Sensitive Detection of Organophosphates. ACS Applied Materials & Interfaces. 2018, 10 (13), p 11125-11134.
23. Vranish, JN; Ancona, MG; Walper, SA; Medintz, IL. Pursuing the Promise of Enzymatic Enhancement with Nanoparticle Assemblies. Langmuir. 2018, 34, p 2901-2925.
24. Malanoski, AP; Breger, JC; Brown III, CW; Deschamps, JR; Susumu, K; Oh, E; Anderson, GP; Walper, SA; Medintz, IL. Kinetic Enhancement in High-Activity Enzyme Complexes Attached to Nanoparticles. Nanoscale Horizons. 2017, 2, p 241 – 252.
25. Breger, JC; Muttenthaler, M.; Delehanty, JB; Thompson, DA; Oh, E; Susumu, K; Deschamps, JR; Anderson, GP; Field, LD; Walper, SA; Dawson, PE; Medintz, IL. Nanoparticle Cellular Uptake by Dendritic Wedge Peptides: Achieving Single Peptide Facilitated Delivery. Nanoscale. 2017, 9, p 10447-10464.
26. Hondred, JA; Breger, JC; Garland, NT; Oh, E; Susumu, K; Walper, SA; Medintz, IL; Claussen, JC. Enhanced Enzyme Activity of Phosphotriesterase Trimer Conjugates on Gold Nanoparticles for Pesticide Detection. Analyst. 2017, 142, p 3261-3271.
27. Turner, KB and Walper, SA. Bacterial Outer Membrane Vesicles: An Emerging Tool in Vaccine Development, as Adjuvants, and for Therapeutic Delivery. Drug Delivery Letters. 2017, 7 (2), p 83 -91.
28. Dean, SD; Turner, KB; Medintz, IL; Walper, SA. Targeting and Delivery of Therapeutic Enzymes. Therapeutic Delivery. 2017, 8 (7), p 577-595.
29. Brown III, CW; Samanta, A; Diaz, SA; Buckout-White, S; Walper, SA; Goldman, ER; Medintz, IL. Dendrimeric DNA Nanostructures as Scaffold for Efficient Bidirectional BRET-FRET Cascades. Advanced Optical Materials. 2017, article number 1700181.
30. Breger, JC; Buckout-White, S; Walper, SA; Oh, E; Susumu, K; Ancona, M; Medintz, IL. Assembling High Activity Phosphotriesterase Composites using Hybrid Nanoparticle Peptide-DNA Scaffolded Architectures. Nano Futures. 2017, 1, 011002.
31. Yuen, JD; Walper, SA; Melde, BJ; Daniele, MA; Stenger, DA. Electrolyte-Sensing Transistor Decals Enabled by Ultrathin Microbial Nanocellulose. Scientific Reports. 2017, 7, article number 40867.
32. Alves, NJ; Turner, KB; DiVito, KA; Daniele, MA; Walper, SA. Affinity Purification of Bacterial Outer Membrane Vesicles (OMV) Utilizing a His-tag Mutant. Research in Microbiology. 2017, 168, p 139-146.
33. Alves, NJ; Turner, KB; Walper, SA. Directed Packaging within Outer Membrane Vesicles from E. coli: Design, Production, and Purification. Journal of Visualized Experiments. 2016, 117, e54458.
34. Alves, NJ; Turner, KB; Medintz, IL; Walper, SA. Protecting Enzymatic Function through Directed Packaging into Bacterial Outer Membrane Vesicles. Scientific Reports. 2016, 6, article number 24866.
35. Liu, JL; Walper, SA; Turner, KB; Brozozog Lee, PA; Medintz, IL, Susumu, K; Oh, E; Zabetakis, D; Goldman, ER; Anderson, GP. Conjugation of Biotin-Coated Luminescent Quantum Dots with Single Domain Antibody-Rhizavidin Fusions. Biotechnology Reports. 2016, 10, p 56-65.
36. Turner, KB; Alves, NJ; Medintz, IL; Walper, SA; Improving the Targeting of Therapeutics with Single-Domain Antibodies. Expert Opinion in Drug Delivery 2016, 13 (4), p 561-570.
37. Field, LD; Walper, SA; Susumu, K; Oh, E; Medintz, IL; Delehanty, JB. Modulation of Intracellular QD:Fluorescent Protein Förster Resonance Energy Transfer via Customized Ligands and Spatial Control of Donor:Acceptor Assembly. Sensors 2015, 15, p 30457-30468.
38. Brown III, CW; Oh, E; Hastman Jr, DA; Walper, SA; Susumu, K; Stewart, MH Deschamps, JR; Medintz, Il. Kinetic Enhancement of the Diffusion-Limited Enzyme Beta-Galactosidase When Displayed with Quantum Dots. RSC Advances 2015. 5, p 93089-93094.
39. Alves, NJ; Turner, KB; Daniele, MA; Oh, E; Medintz, IL; Walper, SA. Bacterial Nanobioreactors – Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles. ACS Applied Materials & Interfaces. 2015, 7 (44), p 24963-24972.
40. Liu, JL; Goldman, ER; Zabetakis, D; Walper, SA; Turner, KB; Anderson, GP. Enhanced Production of a Single Domain Antibody with an Engineered Stabilizing Extra Disulfide Bond. Microbial Cell Factories. 2015, 14:158, p 1-8.
41. Dwyer, C; Diaz, S; Walper, SA; Samanta, A; Susumu, K; Oh, E; Buckout-White, S; Medintz, IL. Chemoenzymatic Sensitization of DNA Photonic Wires Mediated Through Quantum Dot Energy Transfer Relays. ACS Chemistry of Materials. 2015, 27, p 6490-6494.
42. Breger, JC; Ancona, MG; Walper, SA; Oh, E; Susumu, K; Stewart, MH; Deschamps, JR; Medintz, IL. Understanding How Nanoparticle Attachment Enhances Phosphotriesterase Kinetic Efficiency. ACS Nano. 2015, 9 (8), p 8491-8503.
43. Alves, NJ; Turner, KB; Medintz, IL; Walper, SA. Enzyme/Protein Packaged Bacterial Vesicles as an Emerging Therapeutic Delivery Capability. Therapeutic Delivery. 2015, 6 (7), p 873-887.
44. Walper, SA; Turner, KB; Medintz, IL. Enzymatic bioconjugation of nanoparticles: developing specificity and control. Current Opinion in Biotechnology. 2015, 34, p 232-241.
45. Breger, JC; Walper, SA; Oh, E; Susumu, K; Stewart, MH; Deschamps, JR; Medintz, IL. Quantum dot display enhances activity of a phosphotriesterase trimer. Chemical Communications. 2015, 51, p 6403-6406.
46. Samanta, A; Walper, SA; Susumu, K; Dwyer, CL; Medintz, IL. An Enzymatically-Sensitized Sequential and Concentric Energy Transfer Relay Self-Assembled Around Semiconductor Quantum Dots. Nanoscale. 2015, 7 (17), p 7603-7614.
47. Claussen, JC; Malanoski, A; Berger, JC; Oh, E; Walper, SA; Susumu, K; Medintz, IL. Probing the Enzymatic Activity of Alkaline Phosphatase Quantum Dot Bioconjugates. The Journal of Physical Chemistry C. 2015, 119 (4), p 2208-2221.
48. Susumu, K; Oh, E; Delehanty, J; Pinaud, F; Gemmill, K; Walper, S; Berger, J; Schroeder, M; Stewart, M; Jain, V; Whitaker, C; Huston, A; Medintz, I. A New Family of Pyridine-Appended Multidentate Polymers as Hydrophilic Ligands for Preparing Stable Biocompatible Quantum Dots. Chemistry of Materials. 2014, 26, p 5327-5344.
49. Walper, SA; Liu, JL; Zabetakis, D; Anderson, GP; and Goldman, ER. Development and Evaluation of Single Domain Antibodies for Vaccinia and L1 Antigen. PLoS ONE 2014, 9(9), e106263.
50. Liu, JL, Zabetakis, D, Walper, SA, Goldman, ER, Anderson, GP Bioconjugates of Rhizavidin with Antibody Binding Domains as Bifunctional Immunoreagents. Journal of Immunological Methods. 2014, p 37-42.
51. Goldman, ER; Brozozog Lee, PA; Zabetakis, D; Turner, KB; Walper, SA, and Anderson, GP. Negative Tail Fusions Can Improve Ruggedness of Single Domain Antibodies. Protein Expression & Purification 2014, 95, p 226-232.
52. Walper, SA; Battle, SR; Brozozog Lee, PA; Zabetakis, D; Turner, KB; Buckley, PE; Calm, AM; Welsh, HS; Warner, CR; Zacharko, MA; Goldman, ER; and Anderson, GP Thermostable Single Domain Antibody-Maltose Binding Protein Fusion for Bacillus anthracis Spore Protein BclA Detection. Analytical Biochemistry 2014, 447, p 64-73.
53. Walper, SA; Brozozog Lee, P; Anderson, GP; and Goldman, ER. Selection and Characterization of Single Domain Antibodies Specific for Bacillus anthracis Spore Coat Proteins. Antibodies 2013, 2(1), p 152-167.
54. Walper, SA; Brozozog Lee, P; Goldman, ER; and Anderson, GP. Comparison of Single Domain Antibody Immobilization Strategies Evaluated by Surface Plasmon Resonance. Journal of Immunological Methods 2013, 388, p 68-77.
55. Walper, SA; Anderson, GP; Brozozog Lee, P; Glaven, R; Liu, JL; Bernstein, R; Zabetakis, D; Johnson, L; Czarnecki, J; and Goldman, ER. Rugged Single Domain Elements for Bacillus anthracis Spores and Vegetative Cells. PLoS ONE 2012, 7 (3), e32801.
56. Goldman, ER; Anderson, GP; Zabetakis, D; Walper, SA; Liu, JL; Bernstein, R; Calm, A; Carney, J; O’Brien, T; Walker, J; and Garber, E. Llama-derived Single Domain Antibodies Specific for Abrus Agglutinin. Toxins 2011, 3(11), p 1405-1419.
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