Timothy Stasevich Colorado State University Fort Collins
1 protocol

Tatsuya Morisaki
1 protocol

Amanda L. Koch
  • Research associate, Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523
  • 1 Author merit
1 Protocol published
A Multi-color Bicistronic Biosensor to Compare the Translation Dynamics of Different Open Reading Frames at Single-molecule Resolution in Live Cells
Authors:  Amanda L. Koch, Tatsuya Morisaki and Timothy J. Stasevich, date: 07/20/2021, view: 830, Q&A: 0

Here, we describe how to image and quantitate the translation dynamics of a bicistronic biosensor that we recently created to fairly compare cap-dependent and IRES-mediated translation at single-molecule resolution in live human cells. This

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